Powerpoints Flashcards
3 types of research approaches
- exploratory
- correlational
- experimental
exploratory research
gives a sense of variables you should explore more
ex: interviewing people who grow plants in their dorm rooms
correlational research
can show correlation between variables
ex: survey people who grow plants in their room about specific factors
experimental research
can show that one variable causes another
ex: buy plants and conduct experiments on them
negative control
condition we know will produce a negative result
OR condition that will have no change
ex: all plants die
postive control
condition we know will produce a positive result
ex: all plants grow
what do positive and negative controls do?
- ensure that assay is working
2. establish a range of possible results
1 mL =
1000 µL
What must you ALWAYS do before plating microbes?
VORTEX
What must be in each lab notebook entry?
Title, date, names
Purpose
Methods
Data
Conclusions
What side of the plate do you label?
the ager side
What should you label a plate with?
Name
Lab day/time
Date
What is plated (species)
Experimental variables that differ between plate (dilution, treatment)
Dilution formula
C1V1=C2V2
Fold dilution formula
Fold = C1/C2 = V2/V1
Why use a serial dilution?
it is more accurate and saves resources
What is a spot on an ager plate?
a colony
thousands of yeast growing from 1 cell that you plated
How many yeast colonies do you ideally want on a plate?
30-300
Techniques for repairing UV damaged DNA
- photolyase repair
2. nucleotide exision repair
Photolayse repair
specific enzyme senses bulge in DNA and repairs it
Nucleotide excision repair
enzymes cut out the damaged region of DNA
Which technique of DNA repair did placental mammals loose?
photolayse repair
can only use nucleotide excision repair
Qualities to consider when selecting a model organism
- How well they model the organism we are studying
- Ethics
- Ease of growing in lab
- Cost
- Generation time
- Size
- Ease of genetic manipulation
Baker’s yeast scientific name
Saccharomyces cerevisiae
Why use baker’s yeast as a model organism?
easy to grow in lab
2 hours per cell division
30% yeast genes shared with humans
Properties of baker’s yeast
single-celled eukaryote
fungi
can be grown as haploid or diploid
used since egyptians to bake bread