C. elegans methods

Question 1

First step of washing c. elegans

Answer 1

use 1000 microliter M9 buffer 3x

run down plate to scoop c. elegans

Question 2

Do we wash worms with pure water?

Answer 2

no

Question 3

Second step of washing c. elegans

Answer 3

centrifuge test tube

make sure to balance the centrifugeq

Question 4

How long should you centrifuge?

Answer 4

Really quick

a couple of seconds

Question 5

Why not over-centrifuge?

Answer 5

Do not want to squish the worms

Question 6

supernatant

Answer 6

liquid lying about the pellet after centrifuging

Question 7

Third step of washing c. elegans

Answer 7

remove supernatant

add 1ml of M9 onto worms

flick the tube to resuspend the worms

Question 8

Why do we wash?

Answer 8

Do not want to replate bacteria

want to wash bacteria and worm waste away

Question 9

Fourth step of washing c. elegans

Answer 9

centrifuge again after flicking

and remove the supernatant

repeat

Question 10

fifth step of washing c. elegans

Answer 10

resuspend in desired concentration

Question 11

If worms are too concentrated?

Answer 11

Add more solution

Question 12

If worms are not concentrated enough

Answer 12

re-centrifuge and remove supernatant

Question 13

What concentration did we want?

Answer 13

Roughly 40 worms in 5 microliters

Question 14

Benefits of using c. elegans

Answer 14

Hermatphoites

Eat bacteria (cheap)

40% same genes as humans

3 week lifesspan

scientists can manipulate the genome