Posttranscriptional Regulation

Question 1

What kind of bond is made during RNA splicing that produces the lariat structure

Answer 1

2’-5’ phosphodiester bond

Question 2

The Drosophila gene that is activated in XX embryos to product a protein, but not in XY embryos

Answer 2

Sxl gene (sex-lethal gene)

Question 3

The pre-mRNA that is spliced at a downstream 3’ site in XX embryos but at an upstream site in XY embryos because of the Sxl protein

Answer 3

tra pre-mRNA

Question 4

The protein that is produced normally in XX embryos but is nonfunctional in XY embryos because of the alternate slicing of the pre-mRNA

Answer 4

Tra protein

Question 5

The pre-mRNA that is spliced differently in female and male drosophila due to the presence or absence of the Tra protein

Answer 5

dsx pre-mRNA

Question 6

The gene that has a mutation in an intron that affects splicing, causing a problem with blood coagulation factor IX that causes hemophilia

Answer 6

F9 Gene

Question 7

Recognizes primary mRNAs and crops out the stem loop sequences away from the longer RNA

Answer 7

Drosha

Question 8

Cuts the pre-miRNA into a miRNA:miRNA duplex and releases it

Answer 8

Dicer

Question 9

Picks up the miRNA:miRNA duplex and expels one strand

Answer 9

RNA-induced silencing complex (RISC)

Question 10

The mode of RNA interference that occurs when the miRNA and the target mRNA are a perfect match. The miRISC cleaves the mRNA, and RNases rapidly degrade the cleavage product

Answer 10

mRNA cleavage

Question 11

The mode of RNA interference that occurs if miRNA and its target mRNA are not perfect matches. miRISC remains bound to its target and represses ribosome movement

Answer 11

Translational Repression