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PBIO LAB > Post Lab > Flashcards

Post Lab Flashcards

1
Q

Observation for Pineapple glass 3

A

Gelatin does not form liquid

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2
Q

Observation for Pineapple glass 2

A

Gelatin breaks down and forms liquid

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3
Q

Observation for Pineapple glass 1

A

No changes

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4
Q

They are capable of hydrolyzing peptide bonds and are also referred to as peptidases, proteases or proteinases.

A

Proteolytic Enzymes

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5
Q

Proteolytic Enzymes belongs to the hydrolase class of enzymes (T/F)

A

True (Hydrolase)

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6
Q

Action of Proteolytic is essential to:

A

Digestion
Protein Turnover
Cell Division
Blood-clotting cascade
Signal Transduction

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7
Q

Generic name given to the set of derived
endopeptidases belonging to members of the Bromeliaceae family, being able to break the peptide bond, separating proteins and amino acids

A

Bromelain

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8
Q

Scientific name of Pineapple

A

Ananas comosus

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9
Q

Optimum Temperature and pH of enzyme
bromelain

A

50 C
pH 7.0

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10
Q

Egg albumin solution Observation

A

Firm liquid

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11
Q

Beaten egg whites Observation

A

Bubble formation

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12
Q

Heated egg whites Observation

A

Solidifies

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13
Q

Egg albumin solution, alcohol Observation

A

Solidifies

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14
Q

Egg albumin solution and vinegar Observation

A

Solidifies

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15
Q

Refers to the physical changes that take place in a protein exposed to abnormal conditions in the environment.

A

Protein Denaturation

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16
Q

Causes of Protein Denarturation

A

Heat (Temperature)
Acid
Alcohol
Mechanical Agitation
High Salt Concentration

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17
Q

When a protein denatures, its complicated folded structure _______, and it becomes just a __________ of amino acids again.

A

Unravels
Long strand

18
Q

What Protein structures do weak chemical forces hold

A

Tertiary Protein Structures
Secondary Protein Structures

19
Q

It is dependent of its shape, so denatured proteins are no longer functional.

A

Protein Function

20
Q

During cooking the applied heat causes proteins to ________

A

Vibrate

21
Q

This destroys the weak bonds holding proteins in their complex shape

A

Applied (Heat)

22
Q

The unraveled protein strands then stick together, forming an

A

Aggregate (or network)

23
Q

What is biuret reagent made of

A

Copper sulfate CuSO4 (Strong base)

24
Q

Test for peptide bonds

A

Biuret’s Test

25
Q

Positive result for Biuret Test

A

Albumin – violet
Peptone – violet
Gelatin – violet
Casein – violet
Amino Acids – blue

26
Q

How many peptide bonds are required for the formation of violet complex?

A

Two peptide bonds are at
least required

27
Q

Ninhydrin Reacts with ______-

A

Alpha - Amino acids (–NH2)

28
Q

Color given by Ninhydrin when it reacts with α-amino acids

A

purple colored complex

29
Q

In Ninhydrin, Tyrosine and Tryptophan results in a purple color meaning they are?

A

α-amino acids

30
Q

Ninhydrin positive result for Proline and Hydroxyproline

A

yellow

31
Q

Test used to detect amino acids containing an
aromatic nucleus

A

Xanthoproteic Test

32
Q

Positive color result for Xanthoproteic test

A

Tyrosine - Yellow
Tryptophan - Yellow
Phenylalanine - Yellow

33
Q

It is the only amino acid containing a phenol group

A

Tyrosine

34
Q

It is a hydroxyl group attached to a benzene ring

A

Phenol group

35
Q

Test specific for tyrosine, the only amino
acid containing a phenol group,

A

Millon’s Test

36
Q

Positive color result for tyrosine

A

Tyrosine - Brick Red
Phenol Solution - Brick Red

37
Q

specific for free Arginine or proteins
containing Arginine

A

Sakaguchi Test

38
Q

Arginine belongs to what group?

A

Guanidine group

39
Q

Positive color result for Sakaguchi test

A

red-coloured complex

40
Q

Specific for Tryptophan in proteins

A

Hopkins-Cole Test

41
Q

Sulphuric acid in presence of mercuric sulphate
oxidizes the _________ of tryptophan

A

Indole nucleus

42
Q

Positive result for Hopkins-Cole Test

A

violet-purple colored complex

PBIO LAB (7 decks)

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