EX 2 Flashcards
Test done to see if an analyte has peptide bonds or not
Biuret Test
Test for amino acids, and free amino groups
Ninhydrin Test
Test for Tyrosine and Tryptophan
Xanthoproteic Test
Test done for Tyrosine and Phenolic compounds
Millon’s Test
Test for arginine
Sakaguchi Test
Test done for Tryptophan
Hopkin’s Cole Test
Amount of test solution in Biuret Test
1 or 2mL
Amount of biuret reagent
1-2 mL
Procedures for Biuret Test
Take 3 dry and clean test tubes
Add 1 or 2 mL of test solution
Add biuret reagent
Shake and let stand for 5 mins
observe
take note
Procedures for ninhydrin test
Prepare solution with 0.2g in 10 mL water
Add 3 drops of 1% solution of ninhydrin, and 1mL of ethanol then heat for 5 mins to boiling water bath
Positive result for Biuret Test
Blue/Purple
Positive result for Ninhydrin Test
Red, Blue or Purple
Positive result for Xanthoproteic Test
Dark Yellow or Orange
Positive result for Millon’s Test
Red precipitate or solution
Positive result for Hopkin’s Cole test
Purple Ring at the junction
