Polar PIN paper

Question 1

classic models propose direction of auxin transport depends on .. .. localisation of transport components

Answer 1

polar subcellular

-PIN auxin transport facilitators show this, but not sure if can determine direction of auxin flow from this

Question 2

hemagglutinin is an …

Answer 2

epitope

-referred to as HA in this paper

Question 3

in epidermis cells, wildtype PIN2 and PIN2:HA always located at the … side

Answer 3

upper

Question 4

in epidermis cells, wt PIN1:HA observed at .. side

Answer 4

lower

Question 5

in cortex cells both PIN proteins were localised at the … side

Answer 5

basal

Question 6

The polarity of PIN localisation is determined by … specific signals as well as .. specific signals

Answer 6

sequence , cell type

Question 7

PIN1:GFP-3 shown location at the .. side

Answer 7

upper

Question 8

evaluation of root gravitropism confirms PIN2:HA and PIN1:GFP-3 can functionally replace PIN2.

But PIN1:HA and PIN1:GFP-2 can’t

Answer 8

GFP = Green fluorescent protein