PMI02-2007/8 Flashcards
What is pathogenicity?
Ability of a microbe to cause disease, discrete (yes/no)
What is virulence?
Degree of pathogenicity of an organism, continuous (spectrum)
What are virulent bacteria?
Bacteria that usually cause disease when they infect a host
What is a virulence factor/gene?
Bacterial component/gene only involved in pathogenesis
What is a housekeeping gene?
Gene involved in all aspects of a bacterium’s life
List Koch’s Postulates.
- Pathogen occurs in every case of the disease and distribution corresponds to that of lesions observed
- Pathogen does not occur in healthy subjects
- After isolated and repeated growth in pure culture, pathogen can induce disease in susceptible animals
Why do Koch’s Postulates not apply to HIV?
Cannot be grown in pure culture (virus)
Poor animal models
Why do Koch’s Postulates not apply to Helicobacter pylori?
Present in healthy people
Poor animal models
Why do Koch’s Postulates not apply to Streptococcus mutans?
Present in healthy people
Not the sole cause of dental caries
Why do Koch’s Postulates not apply to Chlamydia?
Bacterium cannot be grown in pure culture
Why do Koch’s Postulates not apply to Diphtheria?
Bacterial distribution does not match lesions (systemic disease but organism only found in throat)
Why do Koch’s Postulates not apply to Mycobacterium tuberculosis?
Found in healthy (asymptomatic) people (1/3 of pop)
List the Molecular Koch’s Postulates.
- Disease phenotype should be associated significantly more often with the pathogenic organism than with a non-pathogenic strain
- Specific inactivation of the gene(s) associated with the suspected virulence trait should lead to a measurable decrease in virulence
- Restoration of full pathogenicity should accompany replacement of the mutated gene with the wild-type original
What are virulence genes often encoded on and why is this beneficial for microbes?
Mobile genetic elements
Can be swapped between micro-organisms
Give an example of a mobile genetic element.
Plasmid
Transposon
(Bacteriophage)
What is the main benefit of virulence genes for a microbe?
Improves competitive fitness advantage in host
What are pathogenicity islands?
20-50 genes with low G/C ratio found in genome
Often flanked by mobile elements so can be swapped in their entirety
Encode toxin gene systems and other virulence factors
What virulence genes are often found in plasmids?
Adhesins
Antibiotic resistance
Toxins
Why do bacteria adhere to surfaces?
Prevent rapid clearance/protection
Gain a source of nutrients
What three factors are usually required for a bacterium to cause infection?
Transmission
Adherence
Invasiveness (sometimes)
How could a microbe be transmitted?
Inhalation
Ingestion
Inoculation (needles, skin contact, insects)
Fomite route
What is fomite transmission?
Breathing/coughing/sneezing out droplets onto a surface and someone else touching that surface
What cellular structures can be used by bacteria to adhere to a surface?
Flagellae
Fimbriae
What are found within flagellae and fimbriae which aid adhesion?
Specialised surface proteins involved in:
- direct attachment
- signalling to eukaryotic cell to trigger further adhesion or ingestion
What qualities of a microbe can adhesion affect?
Virulence
Tissue tropism (what it can infect)
Is adhesion always linked to virulence? Why?
No
Long-term commensals need to adhere to surfaces too
What does adhesion of a bacterial to a cell/surface cause?
Change in gene expression within bacteria
Induction of eukaryotic intracellular signalling = changes in gene expression and production of compounds (eg antimicrobial markers, adhesion proteins)
What is colonisation?
Presence of micro-organisms without accompanying disease
What is infection?
Presence of micro-organisms resulting in disease
Describe the changes from contamination to infection.
Initial contamination is just the beginning of colonisation, bacteria not always bound
Become resident micro-organisms on adhesion = colonisation; can stay here or progress further
Division and invasion into deeper tissue = critical colonisation
Gross changes in tissue and host = infection
How can bacteria obtain nutrients from a host?
Soluble waste products or host cell not taking up some nutrients
Released from host cells through damage
What are the two options for a pathogenic bacterium to survive in a host?
Evade immune system
Oppose immune function
What is superoxide dysmutase used for?
By microbes to prevent phagocytosis/damage by oxygen radicals
What is the benefit of being invasive for a microbe?
Penetrate mucosal layers and establish at systemic sites
What is septicaemia?
When a bacterial infection enters the bloodstream
What factors can aid the invasiveness of an organism?
Secretion of bacterial enzymes
Anti-phagocytic factors
Toxins that control host cell uptake mechanisms
Give an example of a bacterial enzyme that can aid the invasiveness of an organism.
Strep. pyogenes = collagenases
Staph. aureus = proteases
Haemophilus leucocidins = coagulases
Give some examples of anti-phagocytic factors that aid the invasiveness of an organism.
Capsule to prevent phagocytosis (Strep. pneumoniae)
M proteins by Group A Streptococci prevent antibodies binding
Fc binding proteins to prevent effector functions
Leukotoxins to kill macrophages