Plus Flashcards

1
Q

Quelles bactéries ont un chromosome linéaire?

A

Streptomyces & borrelia burgdorgferi
Agrobacterium tumefaciens

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2
Q

Impact d’ un chromosome linéaire?

A

Absence de telomérase

Réplication a partir du centre bidirectionnelle
Strepto= protéine terminale séquence inverse “patching” par polymérase

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3
Q

Quelles protéines remplacent les histones chez bac?

A

HU
HN-S
Fis
IHF

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4
Q

ADN peut faire jusqu’à_______x la taille de E coli et est chargé ______________

A

1000
négativement

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5
Q

Condensation = combien de boucles autour d’un centre?

A

30-50

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6
Q

Que font les topoisomérases 1 et 2 dans la condensation

A

Compatibilité+ pour **transcription & réplication & régulation **
Décondensation locale seQ pour accessibilité

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7
Q

Site initiation coli? Nb pb ?

A

OriC 260 pb

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8
Q

Que sont boites DnaA?

A

5 séquences prsq pareilles R1 à R5
9 pb

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9
Q

Pourquoi DnaA,
Est essentielle

A

Réplication + enroulement ADN

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10
Q

Ori c=

A

Site Méthylation pr met. Dam
Région due

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11
Q

Région DUE ?

A

DNA unwinding élément
Riche en at
Machinerie repli s’y insère

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12
Q

Nom de helicase dans initiation réplication chez CoIi?

A

DnaB

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13
Q

Quelle protéine affine pour ADN sb?

A

PASB : inhibe rappariements

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14
Q

DnaG?

A

Primase synthèse amorces Arn

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15
Q

Fin de réplication?

A

Région ter

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16
Q

Protéines de ter chez coli?

A

Tus → ter utilisation substance

17
Q

Protéine ter chez bacillus?

A

R T P : Replication termination protein

18
Q

Protéines ter rôle?

A

Bloquer DnaB = stop fourches

19
Q

Pas de séquence ter =?

A

T de génération ↑
Structure génome ↓ stable
Matériel génétique inchangé

20
Q

Comment on ecrit mutant de ter

A

Δ ter