Pipetting

Question 1

holds a particular volume but does not dispense the exact
volume.

Answer 1

To Contain

Question 2

will dispense the exact volume indicated.

Answer 2

To Deliver

Question 3

Types according to Purpose – measuring or graduated pipets

Answer 3

-Mohr pipet
-Serologic pipet
-Micro pipet

Question 4

does not have graduations to the tip. It is self-draining pipet
but the tip should not be allowed to touch the vessel while the pipet is
draining.

Answer 4

Mohr pipet

Question 5

has a graduation mark to the tip and is generally a
blowout pipet.

Answer 5

Serologic pipet

Question 6

is a pipet with a total holding volume of less than 1 mL.

Answer 6

Micro pipet

Question 7

Automatic pipet’s principle

Answer 7

air displacement

Question 8

Types according to purpose – transfer pipets

Answer 8

Ostwald Folin
Volumetric

Question 9

have a bulb-like enlargement of the pipet stem.

Answer 9

Ostwald Folin

Question 10

is designed to dispense or transfer aqueous solution and is
always self-draining.

Answer 10

Volumetric

Question 11

Function and Drainage of Push button or Micropipets*

Answer 11

F- Deliver a variable or fixed volume
D-Blow out last drop

Question 12

Function and Drainage of Serological, standard pipet

Answer 12

F- Deliver a variable volume

D- Blow out last drop

Question 13

Function and Drainage of Kolmer Serological

Answer 13

F- Deliver a variable volume
D- Drain to baseline

Question 14

Function and Drainage of Mohr

Answer 14

F- Deliver a variable volume
D- Drain to baseline

Question 15

Function and Drainage of Capillary

Answer 15

F- Contain a fixed volume
D- Washout

Question 16

Function and Drainage of Lambda (two types)

Answer 16

F:
a. contain a fixed volume
b. deliver a fixed volume

D:
a. washout
b. blow out

Question 17

Function and Drainage of Ostwald-Folin

Answer 17

F- Deliver a fixed volume
D- Blow out last drop

Question 18

Function and Drainage of
Volumetric, standard type

Answer 18

F- Deliver a fixed volume,

D- Drain by gravity leaving last drop in pipet

Question 19

*Push button pipets can be divided into two types that differ in their mechanics:

Answer 19

air displacement and positive displacement

Question 20

pipets are used for routine measurements

Answer 20

Air displacement pipets

Question 21

have a greater accuracy and precision and are preferred for use with samples having a high viscosity, surface tension, density, or vapor pressure.

Answer 21

positive displacement

Question 22

Cleaning of Glasswares

Presoaking glassware in a ________ water is recommended.

Answer 22

soapy

Question 23

Cleaning of Glasswares

Cleaning solutions:

Answer 23

Potassium dichromate in H2SO4 or HNO3

Question 24

Cleaning of Glasswares

Final rinses:

Answer 24

Type I or II

Question 25

Cleaning of Glasswares

Glassware are sterilized using:

Answer 25

Dry Oven @160 – 180oC (1 and half hours)

*KMnO4 – purple *1 1/2 hour autoclave agar 60’C