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Clinical Chemistry > Automation > Flashcards

Automation Flashcards

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1
Q

meas. of radiant energy absorbed/given off by a molecule after illumination of a light source

A

PHOTOMETRY

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2
Q

should always be present

A

Light source

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3
Q

Class. of Clin Chem Instrumentation that measures EMR:

A

-Photometry

-Electrochemistry

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4
Q

Photometry

A

Spectrophotometry
Emission Flame Photometry/ Filter Photometry
Atomic Absorption Spectrophotometry
Fluorometry
Turbidity & Nephelometry

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5
Q

radiant energy frm short rays (gamma rays) to WL long rays (UV & microwave)

A

EMR (electromagnetic radiation)

  • portions of energy traveling in a wavelength manner
  • shorter wavelength, higher EM energy
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6
Q

Types of EMR:

A

cosmic rays, gamma rays, x-rays, UV rays, infrared, microwaves (radio, tv, radar)

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7
Q

Theory of Light Waves

A
  • photon (E) or discrete packet of energy traveling in waves
  • have a peak/crest & trough
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8
Q

distance bet 2 successive peaks/crests
- gives light its char. color

A

Wavelength

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9
Q

Nanometer (nm)

A

– 10-9m; SI unit

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10
Q

Millimicron (mu)

A

1nm

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11
Q

Angstrom (Å)

A

10-10m, 1nm=10Å

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12
Q

Kinds of wavelength

A
  1. visible spectra – 340-700nm
  2. invisible spectra – UV & IR
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13
Q

distance bet peak & trough

A

Amplitude

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14
Q

assoc. w/ spectro

A

Colorimetry

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15
Q

Colorimetry – assoc. w/ spectro
Primary considerations in analysis:

A
  1. Quality
  2. Intensity
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16
Q

not seen; complimentary to the color seen

A

Color absorbed

17
Q

all colors are transmitted

A

White light

18
Q

Kinds of colorimetry:

A
  1. visual
  2. Photoelectric: Spectro & Filter photometry
19
Q
  • uses eye in determining end pt
  • very crude & subjective; less sophisticated & flexible
  • intensity of color must be proportionate to conc. of the color-producing subs
  • simplest colorimetric analysis using a simple device (comparison of unknown w/ standard)
  • ex: Dubosq colorimeter, Sahli’s hemoglobinometer
A

VISUAL

20
Q
  • refined type of colorimeter
A

Dubosq Colorimeter:

21
Q

Dubosq Colorimeter:
- refined type of colorimeter parts:

A
  1. light source
  2. 2 glass-bottomed cups
  3. 2 movable platforms
    2 glass plungers
  4. optical system
  5. 2 identical scales
    - reading: 20 at platform scales for both cups - left cup: standard sol.

Clinical Chemistry (7 decks)

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