Automation

Question 1

meas. of radiant energy absorbed/given off by a molecule after illumination of a light source

Answer 1

PHOTOMETRY

Question 2

should always be present

Answer 2

Light source

Question 3

Class. of Clin Chem Instrumentation that measures EMR:

Answer 3

-Photometry

-Electrochemistry

Question 4

Photometry

Answer 4

Spectrophotometry
Emission Flame Photometry/ Filter Photometry
Atomic Absorption Spectrophotometry
Fluorometry
Turbidity & Nephelometry

Question 5

radiant energy frm short rays (gamma rays) to WL long rays (UV & microwave)

Answer 5

EMR (electromagnetic radiation)

• portions of energy traveling in a wavelength manner
• shorter wavelength, higher EM energy

Question 6

Types of EMR:

Answer 6

cosmic rays, gamma rays, x-rays, UV rays, infrared, microwaves (radio, tv, radar)

Question 7

Theory of Light Waves

Answer 7

• photon (E) or discrete packet of energy traveling in waves
• have a peak/crest & trough

Question 8

distance bet 2 successive peaks/crests
- gives light its char. color

Answer 8

Wavelength

Question 9

Nanometer (nm)

Answer 9

– 10-9m; SI unit

Question 10

Millimicron (mu)

Answer 10

1nm

Question 11

Angstrom (Å)

Answer 11

10-10m, 1nm=10Å

Question 12

Kinds of wavelength

Answer 12

1. visible spectra – 340-700nm
2. invisible spectra – UV & IR

Question 13

distance bet peak & trough

Answer 13

Amplitude

Question 14

assoc. w/ spectro

Answer 14

Colorimetry

Question 15

Colorimetry – assoc. w/ spectro
Primary considerations in analysis:

Answer 15

1. Quality
2. Intensity

Question 16

not seen; complimentary to the color seen

Answer 16

Color absorbed

Question 17

all colors are transmitted

Answer 17

White light

Question 18

Kinds of colorimetry:

Answer 18

1. visual
2. Photoelectric: Spectro & Filter photometry

Question 19

• uses eye in determining end pt
• very crude & subjective; less sophisticated & flexible
• intensity of color must be proportionate to conc. of the color-producing subs
• simplest colorimetric analysis using a simple device (comparison of unknown w/ standard)
• ex: Dubosq colorimeter, Sahli’s hemoglobinometer

Answer 19

VISUAL

Question 20

• refined type of colorimeter

Answer 20

Dubosq Colorimeter:

Question 21

Dubosq Colorimeter:
- refined type of colorimeter parts:

Answer 21

1. light source
2. 2 glass-bottomed cups
3. 2 movable platforms
   2 glass plungers
4. optical system
5. 2 identical scales
   - reading: 20 at platform scales for both cups - left cup: standard sol.