PIPETTES

Question 1

Type i classification

Answer 1

A. To deliver
B. To contain

Question 2

Type I classification where water is used for calibration

Answer 2

to deliver (TD)

Question 3

Type I classification where Hg is used for calibration

Answer 3

To contain (TC)

Question 4

Classification according to drainage characteristic

Answer 4

TYPE II

Question 4

Type II classification

Answer 4

a. to blow out pipet
b. Self draining

Question 5

information in blow out pippettes

Answer 5

• frosted band/two thin rings
• thicker colored rings

Question 5

Type III classification

Answer 5

a. measuring or graduated pipets
b. Transfer pipets

Question 6

has calibration marks to the tip and is generally a blow-out pipet

Answer 6

serologic

Question 7

does not have graduations to the tip

Answer 7

Mohr

Question 7

may either be a serological or mohr, calibrated either TC or TD. with total holding volume of less than 1 mL

Answer 7

micropipet

Question 7

Printed on the neck of the pipette are the specifications
that indicate:

Answer 7

• maximum volume
• size of division on the pippete
• temp at which calibrations are made
• TD or TC

Question 7

colorless liquid meniscus

Answer 7

lower

Question 8

dark colored miniscus

Answer 8

upper

Question 8

Designed to dispense one volume

Answer 8

Transfer pipets

Question 9

types of transfer pipetes

Answer 9

1. Volumetric
2. Ostwald-Folin Pipet
3. Pastuer pipet
4. Automatic pipets

Question 10

Has a cylindrical bulb located midways between the mouth and the tip

Answer 10

Volumetric

Question 11

has the greates accuracy and precision

Answer 11

Volumetric

Question 12

deliver a single specific volume of liquid, usually
between 1 and 10 ml

Answer 12

Volumetric

Question 13

used for accurate measurement & other nonviscous fluids

Answer 13

Volumetric

Question 14

bulb is located closer to the
delivery tip

Answer 14

Ostwald-Folin Pipet

Question 15

with an etched ring/blow-out pipet

Answer 15

Ostwald-Folin Pipet

Question 16

used to measure viscous fluid

Answer 16

Ostwald-Folin Pipet

Question 17

have no calibration marks and are used to transfer solutions or biologic fluids without consideration of a specific volume.

Answer 17

Pasteur pipet

Question 18

most routinely used

Answer 18

Automatic pipets

Question 19

has a pipetting capability of less than 1 ml

Answer 19

Micropipet

Question 20

dispenses greater than 1 ml

Answer 20

Automatic macropipet

Question 21

3 General Types of Automatic Pipets:

Answer 21

• Air Displacement
• Positive Displacement
• Dispensers and Dilutors

Question 22

relies on a piston for suction creation to draw the sample into a disposable tip that must be changed after each use.

Answer 22

Air Displacement

Question 23

operates by moving the piston in the pipet tip or barrel, much like hypodermic syringe.

Answer 23

Positive displacement

Question 24

Does not require different tip for each use. Requires rinsing and blotting
between samples to prevent carryover.

Answer 24

Positive Displacement

Question 25

automatic pipets that obtain the liquid from a common reservoir and dispense it repeatedly.

Answer 25

Dispensers and Dilutors

Question 26

The dispensing pipets may be bottle –top, motorized, handheld or attached to a dilutor

Answer 26

Dispensers and Dilutors