PHASES OF LAB TESTING (Analytical) Flashcards
specimen analysis steps
- pipet required specimen volume
- prepare PFF (Protein Free Filtrate)
- Add coloring reagent
- incubate mixture
- measure absorbance and percentage transmission using spectrophotometer
components of analytical run
- blank
- standard
- control materials
- unknown
a solution whose main purpose is to set the instrument to zero
blank
types of blank
- air blank
- water blank
- reagent blank
- serum blank
use to calibrate the test
standard
where the CP and CN are cbeing compared to
Standard
a solution that has the same consistensy and component as that of the specimen
control material
an empty cuvette
air blank
cuvette that contains a laboratory or reagent grade water
water blank
water blank absorbance rate
100%
may be used to get rid of any abosorbance reading attributed to the color of the reagent
Reagent blank
eliminates absorbance interference sue to sample discoloration and/or turbidity
serum blank
control should be available in sufficient to last a minimum of _____
1 year
control should be stable for atleast ____
1 year
means freeze drying to preserve the specimen longer
lyophilized
