PHASES OF LAB TESTING (Analytical) Flashcards

1
Q

specimen analysis steps

A
  1. pipet required specimen volume
  2. prepare PFF (Protein Free Filtrate)
  3. Add coloring reagent
  4. incubate mixture
  5. measure absorbance and percentage transmission using spectrophotometer
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2
Q

components of analytical run

A
  • blank
  • standard
  • control materials
  • unknown
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3
Q

a solution whose main purpose is to set the instrument to zero

A

blank

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4
Q

types of blank

A
  • air blank
  • water blank
  • reagent blank
  • serum blank
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5
Q

use to calibrate the test

A

standard

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6
Q

where the CP and CN are cbeing compared to

A

Standard

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7
Q

a solution that has the same consistensy and component as that of the specimen

A

control material

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8
Q

an empty cuvette

A

air blank

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9
Q

cuvette that contains a laboratory or reagent grade water

A

water blank

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10
Q

water blank absorbance rate

A

100%

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11
Q

may be used to get rid of any abosorbance reading attributed to the color of the reagent

A

Reagent blank

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12
Q

eliminates absorbance interference sue to sample discoloration and/or turbidity

A

serum blank

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13
Q

control should be available in sufficient to last a minimum of _____

A

1 year

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14
Q

control should be stable for atleast ____

A

1 year

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15
Q

means freeze drying to preserve the specimen longer

A

lyophilized

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16
Q

sources of control

A
  • pooled control sera
  • commercial control sera
17
Q

pooled control sera should be analyzed how many times to obtain the mean value for each constitituents and the standard deviation

A

20 time

18
Q

acceptable range for a given value of analyte is set at _______

A

+/- 2 SD

19
Q

control that are either prepared drom human serum or from bovine-based products

A

commercial control sera

20
Q

2 types of Systematic Error

A
  • Constant error
  • Proportional error