PHYSICAL EXAMINATION OF URINE Flashcards
encompasses a systematic series of procedures such as physical, chemical, and microscopic examination of urine that are able to be correlated to each other in order to ensure reliable and coherent diagnosis of one’s illness.
Routine urinalysis
is a thorough investigation and documentation of urine’s physical characteristics. It is a crucial step in routine urinalysis procedure because it provides preliminary information about the patient’s health status and is used to confirm or to explain findings in the chemical and microscopic areas of urinalysis.
Physical examination of urine
The __________ of a certain person reflects his/her hydration status.
urine volume
There are several factors that affect urine volume. These factors include:
fluid intake
fluid loss from nonrenal sources
variations in ADH
necessity to excrete dissolved substance
VARIATION IN URINE VOLUME IN A DAY:
Oliguria in infants
<1ml/kg/hr
<0.5 ml/kg/hour
VARIATION IN URINE VOLUME IN A DAY:
Oliguria in adults
<400 ml/day
VARIATION IN URINE VOLUME IN A DAY:
Polyuria in children
2.5-3 ml/kg/day
VARIATION IN URINE VOLUME IN A DAY:
Polyuria in adults
2.5 L
This parameter in the physical examination of urine is not routinely reported, though it is necessary that distinct odor in urine samples received in the laboratory should be notified to attending physicians for the consideration of metabolic disorders or aminoaciduria.
Odor
VARIATIONS OF URINE ODOR
Cause: normal
Aromatic
VARIATIONS OF URINE ODOR
Cause: Bacterial decomposition, Urinary Tract Infection (UTI)
Foul
Ammonia-like
VARIATIONS OF URINE ODOR
Cause: Ketones (diabetes mellitus, starvation, vomiting)
Fruity
sweet
VARIATIONS OF URINE ODOR
Cause: maple syrup urine disease
Maple syrup
VARIATIONS OF URINE ODOR
Cause: phenylketonuria
Mousy
VARIATIONS OF URINE ODOR
Cause: tyrosinemia
Rancid
VARIATIONS OF URINE ODOR
Cause: isovaleric acidemia
Sweaty feet
VARIATIONS OF URINE ODOR
Cause: methionine malabsorption
Cabbage
VARIATIONS OF URINE ODOR
Cause: contamination
Bleach
one parameter used in urinalysis to predict urine concentration and probable dissolved substances present in it
Urine color
Urine color
Normal variations are caused by:
a. Normal metabolic functions
b. Physical activity
c. Ingested materials
d. Pathologic condition
e. Abnormal variations caused by bleeding
f. Liver disease
g. Infection
pigments that are present urine that gives its normal color.
Urochrome
Uroerythrin
Urobilin
- gives the normal yellow coloration of urine.
- It is normally excreted at a constant rate, though an increased production is expected in thyroid disorders and fasting states.
- It also increases when the specimen sits at room temperature.
Urochrome
is a pigment that deposits amorphous crystals in urine upon refrigeration. It attaches itself on amorphous crystals’ surface creating a brick dust appearance especially on amorphous urates.
Uroerythrin
is a derivative of protoporphyrins that come from RBC catabolism. It provides orange-brown color in older urine specimens.
Urobilin
an oxidation product of the normal urinary constituent urobilinogen.
Urobilin
LABORATORY CORRELATION OF URINE COLOR
CAUSE: Recent fluid consumption
CLINICAL/ LABORATORY CORRELATIONS: Commonly observed with random specimens
Colorless
LABORATORY CORRELATION OF URINE COLOR
CAUSE: Polyuria or diabetes insipidus
CLINICAL/ LABORATORY CORRELATIONS: Increased 24-hour volume and low specific gravity
Pale yellow
LABORATORY CORRELATION OF URINE COLOR
CAUSE: D.mellitus
CLINICAL/ LABORATORY CORRELATIONS: Elevated specific gravity and positive glucose test result
Pale yellow
LABORATORY CORRELATION OF URINE COLOR
CAUSE: dilute random specimen
CLINICAL/ LABORATORY CORRELATIONS: Recent fluid consumption
Pale yellow
LABORATORY CORRELATION OF URINE COLOR
CAUSE: concentrated specimen
CLINICAL/ LABORATORY CORRELATIONS: May be normal after strenuous exercise or in first morning specimen
Dark yellow
LABORATORY CORRELATION OF URINE COLOR
CAUSE: B complex vitamins
Dark yellow
Causes colorless urine
Recent fluid consumption
Causes pale yellow urine
Polyuria or diabetes insipidus
Diabetes mellitus
Dilute random specimen
Causes dark yellow urine
Concentrated specimen
B complex vitamins
Dehydration
Bilirubin
Arcriflavine
Nitrofurantoin
Causes orange-yellow urine
Phenazopyridine (Pyridium)
Phenindione
Sulfasalazine (Azulfidine)
Cause yellow-green urine
Bilirubin oxidized to biliverdin
Causes green urine
Pseudomonas infection
Asparagus
Causes blue-green urine
Amitriptyline
Methocarbamol (Robaxin)
Clorets
Indican
Methylene blue
Phenol
Propofol
Familial hypercalcemia
Indomethacin (Indocin Tivorbex)
Causes pink red urine
RBCs
Hemoglobin
Myoglobin
Beets
Rifampin
Menstrual contamination
Causes port wine urine
Porphyrins
Causes red-brown urine
RBCs oxidized to methemoglobin
Myoglobin
Cause brown black urine
Homogentisic acid (alkaptonuria)
Malignant melanoma
Melanin or melanogen
Argyrol (antiseptic)
Methyldopa or levodopa
Metronidazole (Flagyl)
Chloroquine and primaquine
Methocarbamol
Fava beans, rhubarb, or aloe
It pertains to the clarity or turbidity of a urine specimen
Transparency
True or false:
Normally, a urine has a clear transparency or clarity
True
True or false:
Decrease in clarity or increase in turbidity is not always associated to a renal disease or metabolic pathologies
True
➔ View the urine against a white background using adequate room lighting
➔ View through a newspaper print
Urine Clarity Determination
URINE CLARITY PARAMETER
No visible particulates, transparent
Clear
URINE CLARITY PARAMETER
Few particulates, print easily seen through urine
Hazy
URINE CLARITY PARAMETER
Many particulates, print blurred through urine
Cloudy
URINE CLARITY PARAMETER
Print cannot be seen through urine
Turbid
URINE CLARITY PARAMETER
May precipitate or be clotted
Milky
can result in urine that is hazy but normal.
Presence of : squamous epithelial cells and mucus, particularly spx from women
frequently develop a thick turbidity caused by precipitation of amorphous phosphates, carbonates, and urates.
Refrigerated specimens
Produce a white precipitate in urine with an alkaline pH
Amorphous phosphates and carbonates
produce a precipitate in acidic urine that resembles pink brick dust due to the presence of uroerythrin.
amorphous urates
A. Hazy female specimens with squamous epithelial cells and mucus
B. Bacterial growth in
unpreserved specimens
C. Refrigerated specimens with precipitated amorphous phosphates and urates
D. Contamination; fecal talc, semen, vaginal creams, IV contrast media
NON PATHOLOGIC TURBIDITY
A. Most common: RBCs, WBCs, bacteria
B. Nonsquamous epithelial cells, yeast
C. Abnormal crystals
D. Lymph fluid
E. Lipid
PATHOLOGIC TURBIDITY
is determined by the concentration of free H+ ion present in urine sample. As H+ ion increases, pH decreases making the urine acidic while as H+ ion decreases, pH increases bringing alkalinity to urine sample.
urine pH
The lung excretes ___________through respiration, while the kidney reclaims and generates bicarbonate and secretes ammonium ions.
carbon dioxide
is responsible for the bulk of the bicarbonate reabsorption/generation, while the distal tubule provides the remaining function.
proximal renal tubule
may be produced through ingestion of meat, fruits such as cranberries and various pharmacologic agents (ammonium chloride, methionine and methenamine mandelate).
Acidic urine
may be produced after the ingestion of fruits and vegetables (citrus fruits), following a meal ( the so-called alkaline tide), taking medications (Sodium bicarbonate, potassium citrate and acetazolamide).
alkaline urine
Normal urine pH in random sample
4.5 - 8.0
Normal urine pH in first morning urine sample
5.0 - 6.0
Normal urine pH in Nnormal protein diet sample
4.5 - 6.5
Normal urine pH in Unpreserved Urine
9.0
is useful in the evaluation of urine concentration.
● It can determine if the specimen is concentrated enough to provide reliable screening results.
Specific gravity
● It is known as the density of a solution compared with the density of an equal volume of distilled water at the same temperature.
● It is influenced by the number and size of particles present
Specific gravity
URINE SPECIFIC GRAVITY
Hyposthenuric
Isosthenuric
Hypersthenuric
Random specimen
Normal Range
<1.010
1.010
> 1.010
1.015-1.025
1.023
● It is a conventional means to measure urine’s specific gravity
● less accurate than the other methods, it is a simple method that can be done in basic laboratory.
Urinometer
The major limitation of using a urinometer to measure specific gravity is…
it requires a large volume of specimen
Determines the concentration of dissolved particles in a specimen by measuring refractive index.
Refractometer
a comparison of the velocity of light in the air with the velocity of light in a solution.
Refractive index
measures velocity of light in air versus velocity of light in a solution. The concentration of urine changes the velocity and angle at which the light passes through the solution.
Refractometer
The __________ in the refractometer determines the angle that light is passing through the urine and converts angle to calibrated viewing scale
prism
It is a way of determining the urine specific gravity through sound waves of specific frequency that is generated at one end of the tube and as the sound waves oscillate through urine, their frequency is altered by the density of the specimen
Harmonic Oscillation Densitometry
most convenient and most commonly used means of measuring specific gravity in many laboratories worldwide.
Reagent Strip
is based on the change in pKa (dissociation constant) of a polyelectrolyte in an alkaline medium
reagent strip reaction
True or false:
The releasing of H ions is directly proportional to the number of ions in the solution. The more hydrogen ions released, the lower is the pH.
True
Macroscopic hematuria
Red turbid
Red cloudy
Microscopic hematuria
Red color
Clear urine
Blodhein’s test
3.2g Ammonium sulfate + urine = red precipitate
2.8g Ammonium sulfate + urine = clear
Hemoglobinuria
Blodhein’s test
3.2g Ammonium sulfate + urine = red color, no precipitate
2.8g Ammonium sulfate + urine = clear
Myoglobinuria
Urinometer:
In every 3C increase in temp. add?
0.001 in SG
Urinometer
In every 3C<20C, subtract?
0.001 SG
Refractometer
Unless protein is present, subtract?
0.003
Refractometer
Unless glucose is present, subtract?
0.004
Evident pigment when the urine is unpreserved
Urobilin
Most famous urine pigment
Urochrome
Normal color of urine
Pale yellow-datk yellow
Urine color of TB immunocompromised-HIV patient
Red
Presence of __________ causes the urine coffe-black appearance
Methemoglobin
Parameters that measures how concentrated the urine
Specific gravity
Color
Clarity
Most reliable parameter that measures the concentration of urine
Specific gravity
Which method of measuring SG is prone to interferences?
Urinometer
Advantage of using refractometer
Smaller volume of urine is used
No temperature calibration
- metabolite will crystallize
- indicating presence of UTI
Amphicilin & sulfonamide crystals
A contaminant from plastic containers
Talc
Presence of leucine tyrosine and bilirubin crystals are indication of what?
Liver damage