photosynthesis Flashcards

1
Q

Why do plants need both chloroplasts and mitochondria?

A
  • photosynthesis takes place in tissues containing the green pigment chlorophyll
  • these are the chroloplasts; highly structured, membrane rish organelles
  • 40 to 50 chroloplasts per cell
  • chloroplasts convert light energy into chemical energy, and mitochondria consume the chemical energy to produce ATP
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2
Q

What is the purpose of photosynthesis?

A
  • the process of using sunligh to produce carbohydrates
  • requires sunlight, co1, and h20 and produces o2 as a by product
  • usually glucose is produced, but several carbohydrates can be made
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3
Q

What are the two main reaction pathways in photosynthesis and the purpose of each?

A
  • light dependant reactions which produce o2 from h20
  • light independant reactions (cavin cycle) which produce sugar from co2
  • two sets of reactions are linked by electrons: electrons are released in the light-dependant reactions when water is split to form oxygen gas; electrons are transferred to NADP= forming NADPH
  • calvin cycle uses this NADPH to reduce co2: ATP made by the light dependant reactions is also used in the calvin cycle
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4
Q

Would human impacts on the environment affect C3, C4 or CAM plants differently? Why?

A
  • more equipped to survived these conditions
  • global warming causing more hard and dry envionments
  • c4 would thrive

cam
- less sunlight due to co2 and urbanizaition
- cam is temporal

-

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5
Q

summary of photosynthesis

A
  • energy from the sun must be transformed into chemical energy for use by organisms
  • autotrophs are organisms that use sunlight to produce carbohydrates (heterotrophs must meet their needs for nutrients from other oragnisms…. no heterotrophs without autotrophs)
  • electrons carried by NADPH (battery molecule) and the potential energy of ATP link light reactions with co2 reduction to carbohydrates
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6
Q

stsructure of the chloroplast

A
  • the inernal membranes of chloroplasts form flattened, vesicle-like structures called thylakoids, some of which form stacks called grana
  • the fluid-filled space between the thylakoids and the inner memebrane is the stroma
  • light dependant reactions happen in the chloroplasts
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7
Q

light dependant reactions

A
  • starts with chlorophyll
  • chlorophyll molecules work together in groups, foring a complex called a photosystem
  • photosystem 2 feed and ETC that pumps protons to ATP synthases in a process called photorespiration
  • in ps 2 excited eelctrons feed an ETC.
  • plastoquinone carries protons to inside of thylakoids creating a proton motive force. this is analogous to etc in cr, but with diff molecules. similar/same processes
  • protons diffuse down their electrochemical gradient. flow of protons through atp synthase drives the phosphorylation of ADP
  • capture of light energy by photosystem 2 to produce atp is photophosrylation
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8
Q

what is chlorphyll

A
  • chlorophylls are pigments
  • the chlorophylls (a and b) absorb red and blue lght and transmit green light
  • diff pigments absorb diff wavelength of light
  • pigments that absorb blue and red photons are the most effective at triggering photosynthesis
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9
Q

what are photosystems

A
  • two types: 1 and 2 (named after the order in wich they were found)
  • photosystems work together to produdce and enhancement effect in which photosynthesis more than doubles when cells are exposed to both red and far red ligh
  • some organisms only have 1 photosystem, but in organsms with both, photosynthesis is more enchance
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10
Q

how does ps 2 work

A
  • photon hits chlorophyll in ps, a high-energy electron is donated to phenophytin, and chorophyll is oxidized
  • pheophytin is sim to chlorophyll, but is electron acceptor, not donor
  • electron from reduced pheophytin passed to etc in thylakoid membrane
  • etc include plastquinone which shuttles electrons from pheophytin across the thylakoid membrane to a cytochrome complex
  • electrons in etc parpicate in redox rxns. redox rxns result in proons being pumped from one side of membrane to other
  • proton transport increases proton concentration inside the thylakoid 1000 fold
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11
Q

how does ps 2 recharge

A
  • ps 2 splits water to replace its lost electrons and in the process produces oxygen
  • this is oxygenic photosythesis
  • ps2 is the only known protein complex able to oxidize water in this way
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12
Q

how does ps1 work

A
  • reduces nadp+ to nadph
  • excited electrons are passed down an etc of iron and sulfer containing proteins to ferredoxin
  • the enzyme nadp+ reductase transfer a proton and two electrons from ferredoxin to nadp+, forming nadph
  • nadph functions as an electron carrier to reduce other compounds
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13
Q

how does ps1 recharge

A

-ps 2 recharges ps1

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14
Q

what is the z scheme

A
  • model of how ps 1 and 2 interact
  • a photon excites an electron in the P680 chlorophyll of ps2 and passes the eelctron to pheophytin
  • pot energy of the electron is gradually stepped down through redox rxns in an etc
  • PQ uses released energy to transport protons across the thylakoid membrane, builing proton motive force. atp synthase uses this force to make atp
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15
Q

how does z scheme link ps 1 and ps

A
  • at end of ps2 etc, cytochrome complex passes an electron to a protein, plastocyanin
  • pc carries e back to thylakoid membrane and donates it to ps1, linking the two ps
  • e from pc replace e from p700 chlorophyll in ps1. these e enter an etc, passed to ferredoxin and used to reduce nadp+ to nadph
  • e in ps2 are replced by e stripped from water, producing o2
  • explains enhancement effect; most efficeint whn both p680 and p700 avalible
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16
Q

what is the calvin cycle

A
  • common to all photosynthetic eukaryotes (algea to angiosperms)
  • occurs in the chloroplast stroma ( in the cytosol)
  • is a cycle, it spins
  • has three phases: fixation, reduction and regeneration
  • co2 is reduced in the calvin cycle
  • needs three turns of the cycle to generate one G3P
17
Q

fixation

A
  • co2 reacts with ribulose bisphospahe (RuBP), producing two 3-phosphoglycerate molecules
  • the attachement of co2 to an organic compound is called carbon fixation
18
Q

reduction

A
  • the 3-phosophoglycerta molecules are phosphorlate by atp and reduced by nadph to produce glyceraldhyde 3-phosphate (g3p)
  • the 6 g3P produced are used to turn the cycle again
19
Q

regeneration

A

the remaining G3P is used in reactions that regenerate RuBP
- get glucose from regeneration

20
Q

what is rubisco

A
  • ribulose 1,5 bisphosphate carboxylase/oxygenase, or rubisco catalyzes the reaction
  • 10^7 tons of enzyme required globally comprises 40-50% of total solubale lear protin, aka it is very abundant
  • in all photosyntheic organisms
  • worlds most abudant “fixing: protein (fixes approx 200 billiontons o co2 annually)
  • it is the best battle againt the co2 we produce
21
Q

what is the problem with rubisco

A
  • sluggish catalysis enzyme
  • onlt 3 reactions/sec compared to other enzymes which can do thousands/sec
  • it evolved early when the planet had negligable o2 in atmopshere
  • uses o2 as a substrate bu with net loss of c fixes
    d, this is PHOTORESPIRATION
  • cycle used to salvage this carbon uses atp and loses another molecule of co2
22
Q

photorespiration vs calvin cycle

A
  • depends on what molecule is present and what goes to rubisco
  • when o2 is present, co2 is produced = PHOTORESPIRATION. also uses atp
  • calcvin cycle is when co2 is produced
23
Q

what is stomata

A
  • leaf structures where gas exchange occurs
  • open pores bounded by guard cells in plant leaves
  • usually on the underside of leaves because the pore wont get clogged
  • co2 diffuses ino plants through pores of stomata and is fixed by rubisco in mesophyll cells of leaves
  • under dry conditions when plans need to reduce water loss, they close stomatasince co2 and h2o share the same pathway. this also stops plants from fixing co2
  • when co2 conc low during photosynthesis, stomata opens and co2 diffuses in
  • open during day and close at night (open bc water loss)
  • in hot and dry conditions, many plants close stomata toprevent water loss, prvents photosynthesis
24
Q

how can plants get around photorespiration

A
  • plangs in different places have evolved to combat photorespiration in different ways
    c4 plants
  • cam plants
  • c3 plants
25
Q

c4 plants

A
  • carbon fixation and calvin cycle occur in seperate types of cells
  • SPATIAL seperation
  • during C fixation, c4 plants incorperate co2 into 4-c organic acis instead of g3p
  • the acids prodced travel to bundle sheath cells and release a co2 molecule, which rubisco uses to form 3-phosphoglycerate and initates the calvin cycel
  • occurs mostly in plants from hot, dry habitats, limits the damaging effects of photorepiration
  • reduced water loss where water loss if more likely
  • splits up photosynthesis
  • least efficeint
26
Q

cam plants

A
  • in super hot climates
  • cuctus, succulant, etc
  • most effecient compared to c3 and c4
  • reduced water loss if achieved by opening stomata during cool nights and closing thm during the day when temps ar ehigh
  • co2 only enters at night which requires plant to release co2 from stored c4 acids during the day for fixation by rubisco
  • with stomata closed, there is litle o2 present do phororespiration is suppressed
  • uses same spatial as c4. so they run the same thing but it temporally divided
  • SPATIAL and TEMPORAL seperation
  • some cam do both, some only do time
27
Q

c3 plants

A

redular plants

28
Q

Differentiate C3, C4 and CAM plants. Where would you typically find examples of each?

A