Photosynthesis Flashcards
What are the two forms of nutrition ?
1 autotrophic nutrition
2 heterotrophic nutrition
What is autotrophic nutrition ?
Making organic molecules from inorganic raw material obtained from the environment
What are the two types of autotrophs ?
1 photoautotrophs - energy source is light
2 chemo-autotrophs - energy source is chemicals
What is heterotrophic nutrition ?
Organisms that cannot manufacture their own food
What is the equation of photosynthesis ?
6 CO2 + 12 H2O + light energy —chlorophyll—> C6H12O6 + 6 H2O + 6 O2
What are the two stages of photosynthesis ? Where do they occur ?
Light-dependent stage : thylakoid membranes of chloroplast
Light-independent stage : chloroplast stroma
Where are chloroplast found ?
Mainly in mesophyll tissue of the leaf
What are some structures of chloroplast ?
- double membrane
- stroma
- thylakoid membrane
- granum
- thylakoids
- Intergranal lamellae
Description of the chloroplast envelope ?
double membrane
outer membrane highly permeable
inner membrane less permeable with imbedded transport proteins
Function of chloroplast envelope
- allows compartmentalisation and specialisation by enclosing reactant and enzyme involved in photosynthesis together, creates optimal environment for enzymes to function
- special transport proteins in the inner membrane allows regulation of substances moving in and out of chloroplast
What is the lamellae system made up of?
Thylakoids - a flattened disc like sac enclosing a fluid filled thylakoid space, stacked together to form grana
Intergranal lamellae - sheet like pairs of membranes that connects grana
What are the functions of the lamellae system ?
- provides large SA ador attachment of photosynthetic pigments, electrons carriers and ATP synthase : allows for maximum absorption of light, ETC closely located and spatially arranged for transfer of electrons
- compartmentalisation of thylakoid space : allows setting up of proton gradient for ATP synthesis
Description of stroma
Dense like fluid matrix
Contains : circular DNA, RNA, 70s ribosomes, enzymes, starch grains, lipid droplets
Function of stroma ?
- surrounds lamellae system : products of light dependent stage enter Calvin cycle in stroma
- large area for temporary storage of sugars and starch
- DNA codes for some chloroplast proteins, synthesised by chloroplast enzymes
What is the function of photosynthetic pigment ?
To absorb light energy, thereby converting it to chemical energy
Where is photosynthetic pigment located in a plant ?
On thylakoid membranes of chloroplast - usually arranged so that the thylakoid membranes are at the right angles to the light source for maximum absorption
What are the two classes of photosynthetic pigments ( in higher plants ) ?
Chlorophyll
Carotenoids
What light does chlorophyll absorb ?
Mainly red and blue-violet light
Reflects green light
Which photosynthetic pigment is the most abundant ? Why ?
Chlorophyll a
Due to its central role as primary pigment - the only pigment that directly converts solar energy to chemical energy
What is the function of chlorophyll b?
Accessory pigment
Absorbs slightly different wavelengths of light and transfer the energy to chlorophyll a
What are the five structures of chlorophyll related to its function ?
- Long tail is hydrophobic - allows it to be embedded within the phospholipid bilayer of thylakoid membrane
- Head is hydrophilic - allows it to lie at the surface of thylakoid membrane next to the aqueous stroma
- Flat head of chlorophyll molecule lies parallel to membrane surface - maximum light absorption
- Absorption of light by head causes emission of electrons - necessary for initiation of light dependent reaction
- Modification of side groups on the head causes changes to absorption spectrum - diff energies of light absorbed
What light does carotenoid absorbs ? Colour reflected ?
Blue-violet
Reflectes a range of colours, yellow, red, orange, brown but often masked by green chlorophyll
What is the function of carotenoids ?
Accessory pigment and helps absorbs wavelengths of light that chlorophyll a cannot, helps broaden the spectrum of colours that drive photosynthesis
What is a spectrophotometer?
Instrument that measures the ability of a pigments to absorb various wavelengths of light
What is an absorption spectrum ?
Graph depicting the amount of light absorbed by a pigment at different wavelengths of light
What is an action spectrum ?
Record of the rate of photosynthesis occurring at different wavelengths of light
It shows the efficient of different wavelengths of light in photosynthesis
What are the three main reactions of the light dependent stage ?
1 photo-activation of water
2 photolysis of water
3 generation of ATP and reduced NADP
What is the main purpose of light dependent reactions ?
To convert light energy to chemical energy (ATP and red NADP)
What are photosystems ?
Light harvesting clustered arranged at the thylakoid membrane
What are the two types of photosystems ?
Photosystem I (PSI)
Photosystem II (PSII)