phlebo Flashcards
most common anticoagulants
ECHO (edta, citrate, heparin, oxalate)
heparin prevents clotting by
inhibiting thrombin formation
length of the needle should be? butterfly needles?
1- or 1.5-inch
1/2 to 3/4 inch
long cylindrical portion of the needle
shaft
Filling tubes in the wrong order can lead to interference in testing from cross contamination of the specimen by
additive carry over, tissue thromboplastin (present in tissue fluids) & microbial
Using a needle with _____ (what gauge?) increases the chance of hemolyzing the specimen
less than 23 gauge
why cotton balls is not recommended to hold pressure
it tend to stick to the site and reinitiate bleeding when removed because they dislodge the platelet plug that seals a puncture site
substances used to prevent sepsis
antiseptics
Removing the stopper from a specimen can cause
loss of CO2
increase of pH
size of microscope slides used to make blood film for hematology determinations
25 x 75 mm (1 x 3 inch)
tourniquet should be oly used once, for it may transmit what bacteria
MRSA/ methicillin resistant staphylococcus areus
additives for yellow top? what department?
ACD (BB)
SPS (micro)
special tip (needle used for syringe system) that allows the needle to attach more securely than a slip tip
Luer-lock tip
Glycolysis by erythrocytes and leukocytes in blood specimens can falsely lower glucose values at a rate of up to
200 mg/L per hour
most frequently used antiseptics for blood alcohol specimens (2)? what top is used?
povidone iodine
benzalkonium chloride
gray top Na fluoride tube
part of the needle that allows the needle to easily slip into the skin and vein without coring (removing a portion of the skin or vein)
bevel
pressure is maintained ____ when using blood pressure cuff in place of tourniquet
no greater than 40 mm Hg
additives that can be used for green top
Lithium heparin, Sodium heparin, Ammonium heparin
additive for orange top? what department?
thrombin
chem
top/ tube used for blood bank? what additive?
pink top
EDTA
give examples of clot activator
silica
celite
thrombin
substances thatshould be kept at body temp/ 37 C upon collection (3)
cold agglutinin
croglobulins
cryofibrinogen
PTT requires analysis within
within 4 hrs after collection
process of recording in the order received
accession
internal space of the needle
lumen
lenth of the tubing in winged infusion/ butterfly
5-12 inch
use of povidone–iodine to clean skin puncture sites may interferewith tests (4)
bilirubin, uric acid, phosphorus, and potassium
Blood smears made from EDTA specimens must be prepared within
within 1 hour of collection
tubes are handled properly and stored at what temp
4-25 C
substances that should be chilled in crushed ice slurry
ACTH Acetone ACE ammonia catecholamines glucagon lactic acid PTH renin
transfer of blood collected in an additive tube into another additive tube, even if the additives are the same will cause false ____ result
false negative result
3 phlebotomy needles used
multisample needles (evacuated tube system), winged infusion needles (butterfly) & hypodermic needles (syringe system)
tube used for trace element tests, toxicology studies, and nutrient determinations
royal blue top
a portion of a specimen used for testing
aliquot
An easy way to protect a blood specimen from light is to
wrap it in aluminum foil
which is recommended by CLSI, spray-dried EDTA or liquid EDTA
spray dried edta
technique that has been shown to cause aerosol formation
thumb roll
used to hold pressure over the site following blood collection procedures
2- by 2-inch gauze pads folded in fourths
additive for royal blue top? what department?
edta/ Na heparin
chem
anticoagulant used for immunohematology tests such as DNA testing and human leukocyte antigen (HLA) phenotyping
ACD
Glucose test specimens drawn in sodium fluoride tubes are stable for
24 hours at room temperature and up to 48 hours when refrigerated at 2C to 8C
other name for hemoconcentration
venous stasis
Silica particles cause the blood to clot within? in thrombin?
15 to 30 minutes
within 5 minutes
plays a major role in preventing the spread of infection and is an important step in the venipuncture procedure that should not be forgotten or performed poorly
proper hand hygiene/washing
2 patterns of vein
H-pattern
M-pattern
allow for fast, accurate processing, and their use has been shown to decrease laboratory errors associated with clerical mistakes
bar code
substances that should be protected from light
Bilirubin Carotene Red cell folate Serum folate Vitamin B2 Vitamin B6 Vitamin B12 Vitamin C Urine porphyrins Urine porphobilinogen
EDTA specimens for erythrocyte sedimentation rate (ESR) determinations must be tested within
within 4 hours if left at room temperature
PT results on unrefrigerated and uncentrifuged specimens are reliable for up to
24 hrs after collection
routine blood specimens should arrive at the laboratory within
within 45 mins
For antecubital site venipunctures, insert the needle into the skin at an angle of
30 degrees or less
large laboratories have a specific area where specimens are received and prepared for testing
central processing or triage
Interventions to ease pain (2)
EMLA (eutecticp mixture of local anaesthetic) for newborns to adult &
oral sucrose for infants and toddlers
it means to inspect an organ by passing light through its walls?
transillumination; hemoglobin in the blood within the veins absorbs the light, causing the veins to stand out as dark lines
which is preferred for potassium tests, Heparinized plasma or serum?
heparinized plasma; when blood clots, potassium is released from cells into the serum and can falsely elevate result
For a higher degree of antisepsis, the traditional antiseptic is?
povidone–iodine
tubes that are used for clearing or discard purposes only
non additive tubes
recommended dilution for decontaminating nonporous surfaces after cleaning up blood or other body fluid spills in patient-care settings
1:100 dilution of Na hypochlorite
most common antiglycolytic agent
Na fluoride
stoppage of blood flow during the blood draw can result in an underfilled tube called
short draw
citrate prevents coagulation by? how many inversions?
chelating calcium
3-4 inversions
additive in special erythrocyte sedimentation rate (ESR) tubes with black stoppers
Na citrate
dilution applied prior to cleanup?
contact time required for disinfectants to be effective?
1:10 dilution
10 minutes
give examples of antiseptics used for blood collection
70% Ethyl alcohol
70% Isopropyl alcohol (isopropanol)
Benzalkonium chloride (e.g., Zephiran chloride)
Chlorhexidine gluconate
Hydrogen peroxide
Povidone–iodine (0.1%–1% available iodine)
Tincture of iodine
chemical substances used to remove or kill microorganisms on surfaces and instruments?
most prefered?
disinfectants
5.25% sodium hypochlorite
Evacuated tubes are available from a number of different manufacturers and come in various sizes and volumes ranging from
1.8 to 15 mL
part of the needle where the blood collection device is atttached
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