Phase 1 - Week 9 (GI tract, Digestion, Eating Disorders) Flashcards
List the classes of food
- Carbohydrates
- Proteins
- Fats
- Vitamins
- Minerals
Describe the types of carbohydrates
- 3 carbons = triose
- 4C = tetrose
- 5C = pentose
- Sugars linked by glyosidic bonds to form complex glycans
- Saccharides - homoglycans, single suger/heteroglycans, complex composition
How do carbohydrates provide energy?
Broken down into glucose, then undergo glycolysis:
Glucose -> glucose-6-phosphate (2ATP -> 2ADP)-> fructose-6-phosphate -> fructose-1,6-biphosphate -> glyceraldehyde-3-phosphate ->->->->-> 2 x pyruvate (NAD, Pi -> NADH and 4 ADP -> 4 ATP)
In the presence of oxygen, the products of glycolysis then go on to complete the citric acid cycle which is followed by the electron transport chain.
Overall, aerobic respiration fuelled by one molecule of glucose gives a net production of 38 ATP.
Where are lipids found?
In plasma, adipose tissue and biological membranes
How do fats provide energy?
Fats are released from triglyceride stores in adipose tissue (with glycerol) via action of lipase enzyme - short chains can enter cells directly, long chains go along carnitine shuttle.
Fats -> fatty acids and glycerol
Fatty acids -> acetyl coA by beta oxidation
Acetyl coA can be used in the Krebs cycle (follows normal aerobic respiration).
How do proteins provide energy?
Broken down into amino acids then alpha ketoglutarate by transamination then oxidative deamination.
Transamination
Converts one amino acid into a different amino acid
Oxidative deamination
An amino group is lost from an amino acid, converting it into alpha ketoglutarate so that it can enter the Krebs cycle
Why do cells require energy?
- Contraction of muscle cells
- Accumulation of ions + other molecules against concentration gradients (e.g. for nerve impulse transmission + control)
- Biosynthesis - building of tissue
- Waste disposal - getting rid of end products of bodily function
- Generation of heat and maintenance of body temperature
Which enzymes are involved with glycolysis?
- Fructose-6-phosphate -> fructose-1,6-biphosphate mediated by phosphofructokinase
- Glyderaldehyde-3-phosphate -> 1,3-biphosphoglyceric acid mediated by phosphate dehydrogenase
Describe the energy investment stage of glycolysis
Glucose -> fructose-1,6-biphosphate = energy investment stage, as 2 ATP are converted to 2 ADP
Describe the energy payoff stage of glycolysis
Glyceraldehyde-3-phosphate -> 2 pyruvic acid = energy payoff stage as 4 ADP are converted to 4 ATP and NAD is converted to NADH
Describe the process of acetyl coA formation
Pyruvic acid -> acetyl coenzyme A (CO2 lost, coA added)
also,
NAD+ -> NADH
Describe the process of lipolysis
- Triglyceride -> glycerol and 3 fatty acids
- Glycerol -> glyceraldehyde-3-phosphate, then continues through glycolysis or undergoes glucogenesis to form glucose + 2 pyruvate
- Fatty acids undergo beta oxidation to form acetyl coA + NADH + FADH2
Describe the Krebs cycle
Acetyl coA + oxaloacetate -> citrate -> isocitrate -> alpha-ketoglutarate (5C) -> succinyl coA (4C) -> succinate -> fumarate -> malate -> oxaloacetate
What is the overall yield of the Krebs cycle?
- 2ATP from GTP
- 6 NADH
- 2 FADH2
- 2 CO2
List the enzymes involved with the Krebs cycle
- Citrate -> isocitrate, enzyme is acontinase
- Isocitrate -> alpha-ketoglutarate, enzyme is isocitrate dehydrogenase
- Alpha ketoglutarate -> succinyl coA enzyme is alpha ketoglutarate dehydrogenase complex
Describe the steps involved in the electron transport chain
- NADH gives 2 electrons to NADH dehydrogenase complex, NADH oxidised to NAD+
- 2 electrons pass to coenzyme Q, 2 hydrogen protons pumped from mitochondrial matrix into space between inner + outer mitochondrial membranes
- Coenzyme Q shuttles elevtron to cytochrome b-cl complex - pumps electrons across membrane
- Electrons return to mitochondrial matrix, combine w/ oxygen atom + 2 hydrogen atoms, 2 hydrogens pumped across membrane (oxygen = final electron acceptor)
- Oxidative phosphorylation - protons passing through matrix allows ATP synthase to synthesise ATP
How is FADH2 used to produce ATP
Follows similar pathway to NADH - bypasses first complex straight to coenzyme Q
Describe glycolysis in anaerobic respiration
Glucose converted to 2 pyruvate by same process as aerobic, pyruvate converted to lactate in reversible step (will be turned back to pyruvate when oxygen is present)
Summarise the number of ATP produced in each stage of respiration
Glycolysis =
2 ATP from substrate level phosphorylation
4-6 ATP from oxidative phosphorylation of 2NADH + H+ in ETC
Acetyl coA formation =
6 ATP from oxidation of NADH + H+ in ETC
Krebs Cycle + ETC =
2 ATP from conversion of GTP -> ATP
18 ATP from oxidative phosphorylation of 6 NADH + H+ in ECT
4 ATP from oxidative phosphorylation of 2 FADH2 in ECT
Give the total net yield of ATP from aerobic respiration
34-36 ATP (theoretical)
29-30 ATP (realistic)
Describe the path followed by food during digestion
- Oral cavity
- Pharynx
- Oesophagus
- Stomach
- Small intestine
- Large intestine
Describe digestion of food in the oral cavity
- Salivary glands - saliva moistens food and amylase in saliva breaks down starch
- Chewing = mechanical digestion
Describe the structural features of the pharynx involved with digestion
- Lined with mucosa to protect from friction caused by swallowing
- Has superior, middle and inferior pharyngeal constrictors to perform sphincteric and peristaltic actions during swallowing
Describe the structural features of the oesophagus involved with digestion
- Lined with lamina propria, muscularis mucosae and stratified squamous epithelium - protects from extreme temperatures, chemicals and abrasion from food particles
- Upper oesophageal sphincter - opens to allow food bolus to enter and closes to prevent regurgitation into the pharynx , under voluntary control
- Lower oesophageal sphincter - formed by portion of the diaphragm, constricts during respiration to prevent regurgitation into the oesophagus, under involuntary control
Describe the histology of the oesophagus
- Non-keratinised stratified squamous
- Lamina propria
- Muscularis mucosae
- Submucosa
- Muscularis propria (inner circular, outer longitudinal)
- Adventitia
Describe the histology of the stomach
- Same as oesophagus but has a 3rd muscular layer (oblique muscular layer)
- Chief cells - secrete pepsinogen
- Parietal cells - secrete hydrochloric acid + intrinsic factor
- G (gastric) cells - secrete gastrin
- Mucous neck cell - secrete mucus
List the functions of the stomach
- As a mixing compartment, breaking up food (mechanical digestion) and mixing it into chyme (a semi-fluid pulp)
- Storage of partially digested food - food is eaten much faster than body can digest/absorb it
- Secretes gastric juice containing water, mucus, hydrochloric acid, intrinsic factor and pepsinogen which contributes to the chemical digestion process
Describe the regions of the stomach
From oesophagus -> duodenum
- Cardiac region
- Fundus
- Body
- Pyloric antrum
- Pyloric canal
- Pyloric sphincter
- Pylorus
How does the stomach contribute to mechanical digestion?
Peristaltic movements created by the 3 layers of smooth muscle - longitudinal layer, circular layer and oblique layer
Parietal cells of the stomach
- Produce hydrochloric acid - denatured ingested proteins, activates digestive enzymes (e.g. pepsinogen) and kills microorganism
- Also produce intrinsic factor which aids the absorption of vitamin B12 from the small intestine
Chief cells of the stomach
- Major producers of digestive enzymes e.g pepsin
- Pepsin is secreted as inactive pepsinogen, converted into active pepsin by hydrochloric acid
- Also secretes gastric lipase which breaks down lipids
- Lingual lipase (in saliva) is activated by the acidic environment of the stomach
Mucous neck cells of the stomach
Produce mucus, which helps to protect the lining of the stomach from hydrochloric acid and digestive enzymes
List the accessory glands/organs of the digestive system
- Pancreas
- Liver
- Gallbladder