Pharmacoproteomics

Question 1

What is the P4 model of healthcare?

Answer 1

• Predictive
• Personalized
• Preventive
• Participatory

Question 2

What are the benefits of P4 medicine?

Answer 2

• Provide more cost effective disease care
• Reduce incidence of care
• Replicate the innovation cycle of systems medicine on a large scale, as both disease care and wellness support are integrated with discovery science, thereby creating what is called a “learnig healthcare system”

Question 3

What is systems biology?

Answer 3

It is the study of biological systems as collections of networks at multiple levels, ranging from the molecular level, through cells, tissues and organisms, to the population level

Question 4

What is systems medicine?

Answer 4

It is the medical application of systems biology, a merger of molecular biology, mathematical remodelling, and systems theory

Question 5

What is pharmacoproteomics?

Answer 5

It deals with the changes in the abundances of the proteins caused by the administration of the medications, and therefore examines at the protein level

Question 6

What does the “omics” stand for in the word proteomics?

Answer 6

• Sub-typing of patients based on the basis of protein analysis
• Matching a particular target-based therapy to a particular marker in a subgroup
• Matching a particular target-based therapy to a particular marker in a subgroup (can help develop multi-target drugs)
• Can help provide optimal detection of disease at an early stage for prevention and early intervention

Question 7

What is an immunoassay?

Answer 7

It is a highly selective bioanalytical method that measures the presence or concentration of analytes (small and large macromolecules) through the use of an antibody or an antigen as a biorecognition agent

Question 8

What are the three types of immunoassays?

Answer 8

1. Direct Detection Immunoassay (the antibody directly binds to the surface)
2. Indirect Detection Immunoassay (the secondary antibody binds to primary antibody, which in turn is bound to the target)
3. Sandwich Immunoassay (Antibody binds to immobilized antibody on target)

Question 9

What is mass spectroscopy?

Answer 9

MS is a technique in which gas phase ions are separated and detected according to their mass-to-charge ratio

Question 10

What is toxicoproteomics?

Answer 10

It seeks to determine how chemical exposure modifies proteins or protein expression as a form of preclinical risk assessmentRe

Question 11

Review slides 20-22