Pharmacogenetics Flashcards
4 different drug responses?
toxic + beneficial
toxic + not beneficial
non-toxic + beneficial
non-toxic + non-beneficial
3 goals for personalized medecine?
- Identify genetic differences between people that affect drug response
- Develop genetic tests that predict an individual’s response to a drug
- Tailor medical treatments to the individual
Difference between pharmacogenetics and pharmacogenomics?
genetics : evaluate how an individual’s genetic makeup corresponds to their response to a particular medication
genomics : large group of patient to evaluate how drugs interact with many different genes and their proteins
5 drugs that are ineffective in a certain percentage of people?
- ACE inhibitors - Hypertension (10-30%)
- Beta blockers - Heart Failure (15-25%)
- Anti-depressants (20-50%)
- Statins - Cholesterol (30-70%)
- B2 agonists - asthma (40-70%)
How can a change in a gene lead to toxicity/low efficacy in PK? (3)
Gene that affects ADME
Drug metabolizing enzymes
T.I.
How can a change in a gene lead to toxicity/low efficacy in PD? (1)
Slightly different target or loss of target
The two major types of drug metabolizing enzymes? What do they act on? (2)
Ultra drug metabolizer
Poor drug metabolizer
They act on drugs and pro-drugs
Effect of ultra drug and poor drug metabolizers on drugs?
UM : rapid inactivation so ineffective
PM : toxic doses
Effect of ultra drug and poor drug metabolizers on prodrugs?
UM : metabolized to active form rapidly, toxic doses
PM : Little conversion of prodrug to active form, ineffective
Three reasons why there can be NO consequences of genetic polymorphisms?
- Outside of coding and regulatory regions
- Synonymous substitution
- No signi. effect on function
4 consequences of genetic polymorphisms?
None
Decrease/loss function of protein
Increase function of protein
Change is compared to population majority
What are 4 reasons why there can be decreased function in a protein due to genetic polymorphisms?
- Less enzyme produced
- Enzyme not complete (stop codon)
- Enzyme not stable
- Less affinity for substrate
What are 3 reasons why there can an increased function in a protein due to genetic polymorphisms?
- More production
- More stable
- More affinity for substrate
3 enzymes with genetic polymorphisms that cause disease?
NAT2
TPMT
ALDH2
NAT2 involved in what disease?
Tuberculosis
Tuberculosis is an infection of which bacteria
mycobacterium tuberculosis
Tuberculosis attacks mainly what?
lungs
Which drug targets TB? It is metabolized by the _____ via which modification?
When the drug reaches toxic levels it causes what?
Isoniazid
Liver
Acetylation
Peripheral neuropathy
Which enzyme acetylates isoniazid?
NAT2 : N-acetyl-transferase
Two types of NAT2 acetylators? Which is WT and which has many AA substitutions?
Rapid: WT
Slow : many alleles
11 haplotypes of NAT2? Rapid or slow?
4 : rapid 5A : slow 5B : slow 6A : slow 7B : slow 7Bnew : unknown 10 : unknown 11 : unknown 12A : rapid 13 : rapid 14B : slow
Thiopurines treat which diseases? (4)
- Leukemia
- Inflammatory bowel disease
- Prevent organ rejection
- Other autoimmune diseases