Adjuncts and Pain Flashcards
Transient Receptor Potential Channels (TRPs) : how many families? They are the molecular sensors of what?
6 families
Molecular sensors of taste, touch, temperature and pain
What are the 6 families of TRPs?
TRPVs (Vanilloids) TRPA (Ankyrin) TRPPs (Polycistin) TRPCs (Canonical) TRPMs (Melastatin) TRPMLs (Mucolipin)
Which TRP is sensitive to garlic, horseradish and cinnamon?
TRPA1
Which TRP is sensitive to Mint?
TRPM8
Which TRP is sensitive to BAA?
TRPV4
Which TRP is sensitive to Camphor?
TRPV3 and TRPV1
Which TRP is sensitive to Chili and garlic on top of camphor?
TRPV1
Put these TRPs in order of who is sensitive to the coolest to the hottest temperature :
TRPM8, TRPV4, TRPV3, TRPV2, TRPA1, TRPV1
Coolest TRPA1 TRPM8 TRPV4 TRPV3 TRPV1 TRPV2 Hottest
What is the hot component of chilli peppers? Where do they act?
Capsaicin
They act of sensory nerves
What is the MOA of Capsaicin?
Initial sensitization of the nerve, then a prolonged desensitization
What type of receptor is the capsaicin receptor? Where are they expressed?
TRPV1
Expressed on C-fibres and A-delta fibres
6 activators of TRPV1?
Capsaicin Endogenous agonists Acidosis Heat Na Ca
Is TRPV1 a selective or non-selective cation channel?
non-selective
Endogenous ligands of TRPV1? (2)
Exogenous ligands? (2)
Anandamine
Endovanilloids
Capsaicin
Resiniferatoxin
What happens when acute low concentrations activate TRPV1s?
Ca-dependent phosphorylation of ion channels, depolarization and action potential into the sensory neuron and pain, eventual desensitization
What happens when prolonged exposure or high concentrations act on TRPV1s?
Ca overload, mitochondrial dysfunction, destruction of nerves (C and A-delta fibres)
Two therapeutic potentials of TRPV1?
What are the adverse effects?
Topical capsaicin creams
8% capsaicin patch
Effects: skin irritation, burning, oedema
Example of a TRPV1 antagonist? What does is treat?
Low potency or high potency?
Selective or non-selective?
Why did it get withdrawn from clinical trials?
AMG517, treats inflammatory pain
Highly potent
Selective
Withdrawn because is caused concentration-dependent hyperthermia that lasted days and blocked the thermoregulatory centres in the CNS
TRPM8:
Selective or non-selective?
Where is it expressed?
Expression increases in models of _________ _____
Non-selective
Expressed in 15% of small diameter sensory neurones
Expression increases in models of neuropathic pain
What are 3 exogenous ligands for TRPM8?
Menthol
Icilin
Spearmint
Which cannabinoids are derived from the Cannabis plant? Which are man-made? Which are present naturally in the body?
Plant: Phytocannabinoids
Man-made: Synthetocannabinoids
Naturally in the body: Endocannabinoids
What are the two cannabinoid receptors? Where are they located?
CB1 : On central and peripheral nerves
CB2 : Associated with immunocytes
How do CB1 agonists given in the peripheral nerve affect Joint Mechanosensitivity?
It decreases firing frequency in neurons, essentially diminishing action potential
Dorsal horn of the spinal cord : How do cannabinoids act in this location?
- CB1 receptors are activated
- Opening of K ion channels, increase in potassium conductance to the primary afferent neuron, causing it to hyperpolarize
- Closes Ca channels
- Decreases excitatory NT release
What are the three sites of action of cannabinoids?
Peripheral nerve
Dorsal horn of the spinal cord
Suprapinal Regions
What do cannabinoids target in the suprapinal regions? (4)
Cortex
Amygdala
Periaqueductal gray
Rostral ventromedial medulla
what are the periaqueductal gray and the rostral ventromedial medulla involved in?
Descending inhibition
When cannabinoids act of the cortex, what does this cause?
Alters cognitive perception of pain
When cannabinoids act on the amygdala, what does this cause?
Alters emotional perception of pain
Which endocannabinoids are synthesized from phospholipids and Arachidonic acid? (5)
- Anandamide
- Noladin
- Virodhamine
- 2-AG: 2-Arachidonoylglycerol
- NADA: N-Arachidonyl Dopamine
what is the process of anandamide degradation?
It interacts with CB receptor, Anandamide membrane transport rapidly transports it into the cell where FAAH degrades it into ehtanolamine and AA
How do you inhibit the degradation of endocannabinoids?
block the enzymes that cause degradation
Which drug inhibits FAAH from degrading anandamine?
URB597
What are the effects of inhibiting FAAH on OA Joint mechanosensivity?
Decrease in neuronal firing
Describe leukocyte trafficking in an inflamed joint
Leukocytes usually travel freely in vascular space.
A signal from the endothelium is made when there is inflammation
Leukocytes undergo a rolling process to cross the membrane into the extravascular space
Releases mediators once in the specific tissue
What 6 diseases use medical cannabis? %?
Arthritis 36% Spinal Cord Injury 16% AIDS/HIV 15% MS 14% Spinal Cord Disease 10% Cancer 6% Epilepsy 3%