Phagocytosis Flashcards

1
Q

Process by which specialized cells engulf and destroy foreign particles such as microorganisms or damaged cells.

A

Phagocytosis

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2
Q

Most important phagocytic cells

A

Macrophages and Neutrophils

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3
Q

A cell that ingests microorganisms and foreign particles.

A

Phagocytic cells

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4
Q

Extremely small, relatively simple prokaryotic microorganisms traditionally classified with the fungi as Schizomycetes.

A

Bacteria

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5
Q

Something that suggests the presence or existence of a fact, condition, or quality

A

Signs

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6
Q

Any indication of a disease or injury perceived by the patient

A

Symptoms

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7
Q

The site of the experiment should be..

A

Sterilized

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8
Q

Blood should be drawn by an…

A

Aseptic technique

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9
Q

A minimum of 2 ml of blood is put into the

A

Green-top evacuated tube

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10
Q

A green top tube has what kind of anti-coagulant

A

Heparin

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11
Q

(True or False)
The microscope shouldn’t be placed on a plain, stable surface.

A

False

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12
Q

(True or False)
The voltage of the power supply should be inclined with that of the equipment.

A

True

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13
Q

What organism should we use in this experiment

A

Bacillus subtilis or Staphylococcus epidermidis

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14
Q

What stain is used in the experiment

A

Wright stain

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15
Q

How many drops of buffy coat should be added

A

4-8 drops

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16
Q

How many drops of bacterial broth culture is added?

A

2-3 drops

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17
Q

(True or False)
Place 2 drops of the incubated specimen on a glass slide and prepare a smear.

A

False

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18
Q

(Interpret)
Demonstration of the engulfment of bacteriaz

A

Positive

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19
Q

(Interpret)
No engulfment of bacteria

20
Q

(True or False)
The procedure may produce false-negative results if the blood specimen is not fresh.

21
Q

(True or False)
Coagulase-positive Staphylococcus specimen may produce false-positive results.

22
Q

(Interpret)
Bacteria is not intracellular

23
Q

(True or False)
The bacteria must be intracellular and not extracellular for the test to be positive.

24
Q

The failure of phagocytes to engulf bacteria can support the diagnosis of what?

A

Neutrophilic dysfunction

25
Action of a specialized group of phagocytic cells that provide a defense mechanism against invasion by engulfing foreign particulate matter.
Phagocytosis
26
Constitute the major cellular components (mast cells, neutrophils, macrophages)
Phagocytes
27
Activated phagocytes have increased surface receptors that allow for adherence to the bacterium
Initiation
28
Process in which cells tend to move in a certain direction under the stimulation of chemical substance.
Chemotaxis
29
Shows positive chemotaxis
Leukocytes
30
Away from the stimulating substances
Negative Chemotaxis
31
Toward the stimulating substances
Positive Chemotaxis
32
By active ameboid motion; final structure is known as vacuole/phagosome; bacteria must be hydrophobic than the phagocyte
Engulfment
33
Interact with the surfaces of bacteria, rendering them acceptable to the phagocyte
Opsonin
34
Engulfment of antigen
Phagosome
35
Fusion of phagosome and lysosome
Phagolysosome
36
Minute cell particles that contain certain hydrolytic enzymes and peroxides approach the phagosome, fuse with it, rupture, and discharge content to it.
Digestion
37
Has defensins and cathepsin-G
Oxygen independent
38
Formation of superoxide ---> hydrogen peroxide ---> hypochlorite
Oxygen dependent (oxidative burst)
39
Physical contact between the phagocytic cell and the microorganism occurs, aided by opsonins.
Adherence
40
Outflowing of cytoplasm to surround the microorganism
Engulfment
41
Microorganism is completely surrounded by a part of the cell membrane.
Formation of phagosome
42
Lysosomal granules contact and fuse with the phagosome.
Granule contact
43
Contents of the lysosome are emptied into this membrane-bound space.
Formation of phagolysosome
44
Uses hydrolytic enzymes
Digestion of microorganisms
45
Contents of phagolysosome are expelled to the outside by exocytosis
Excretion