Performing Surface Sampling

Question 1

What is step 1?

Answer 1

Perform garbing and handwashing in the ante room before entering the buffer area

Question 2

What is step 2?

Answer 2

Remove the cover of the contact sampling device aseptically and press firmly to the surface being sampled. Cover the contact material back up.

Question 3

What is step 3?

Answer 3

Invert the plates and incubate the contact sampling devices at 20°C to 25°C for 5 to 7 days and then at 30°C to 35°C for 2 to 3 additional days.

Question 4

What is the final step?

Answer 4

Examine the sampling devices for growth daily during normal business hours, and record the observed count at each time point. At the final time point, record the total number of discrete colonies of microorganisms (CFU/sample) on the environmental sampling record based on sample type, sample location and sample date.