PEPCK mech and structure

Question 1

PK

Answer 1

To go around, must do two steps…

Question 2

CO2 lost in 1st step

Answer 2

this is the one originally added to pyruvate. Does not matter because its already a by product???/

Question 3

What catalytic types and o chem mechs?

Answer 3

SN2-like
prox and orientation
ES (Mg is electron soak, coordinated to two terminal Pi and CO2 that was lost.

Question 4

human PEPCK

Answer 4

GTP specificity binding pocket. Carbonyl of GTP is able to have H bonds with surrounding amino acids.

H bonds to amino acids in binding pocket which allows to specifically choose GTP over ATP