PCR YOunger Flashcards
What is DNA amplification done completely in a tube.
PCR
What amplifies fragments by inserting them into a vector and the host replicating the vector.
DNA cloning
a DNA polymerase uses dNTPs to extend the primer and build a complementary copy of the DNA strand
Chain extension
DNA sample is cooled and the primers bind to the complementary sequence in the target DNA
anneal the primers
Produce single stranded DNA
Heat is used
Denature the target DNA
What are temps with the steps?
Denaturization– 95 C
Annealing– 55 C
Extension 72 C
What are some advantages of PCR?
sensitivity- only need trace amounts
speed- only a few hours
DNA can be used for gel electrophoresis
Some uses- mutation detection, detection of latent viruses (HIV), forensics, prenatal genetic diagnosis
What kind of Blot uses DNA?
Southern blot
What kind of Blot uses RNA?
Northern blot
What kind of blot uses proteins?
Western blot
What is a good way to qualitate mRNA levels, (tells how much cancer is in a pt) ?
Microarrays
What is similar to genomics but looks at all the different proteins produced in particular cell or tissue types
Proteins/ Proteomics
What is an extremely sensitive test, that if you get a negative you know you don’t have it?
ELISA
If you run an ELISA and want to make sure your indication was true then you could run a ___________ to make sure it wasn’t a false positive.
western blot
The reason you would expect to see an RFLP
gene crossover, enough DNA getting moved around on the chromosomes there is a passenger polymorphism.
