PCR Flashcards
1
Q
Intro To Nucleic Acids
A
2
Q
Def of PCR
A
An enzymatic method, Used for making multiple copies of a pre-selected segment of DNA /RNA.
3
Q
RNA Extraction
A
3
Q
what are The primary materials (reagents) used in PCR?
A
4
Q
what should happen before PCR?
A
We need to extract the nucleic acid from the cell at first.
4
Q
DNA Extraction
A
5
Q
Cyclic reactions
A
- There are 3 major phases, which are repeated for 20 to 40 cycles.
- This is done on an automated Thermal Cycler.
5
Q
Phases of Cyclic reactions
A
6
Q
Verification of PCR products
A
- Agarose gel electrophoresis.
- Hybridized with a labelled probe.
7
Q
Clinical applications of PCR
A
