PCR Flashcards
1
Q
First step
A
Denaturing- heat to break H bonds holding DNA together (unzip DNA)
2
Q
Second step
A
Annealing- primers bind to DNA at specific sequence
3
Q
Third step
A
Extending - polymerase enzymes begin adding complementary free nucleotides to the template stands
4
Q
Ingredients needed for PCR
A
DNA sample Primers Nucleotides Taq polymerase Mix buffer PCR tube
5
Q
Temperatures at each step
A
95
55
72
6
Q
What machine calls PCR
A
Thermal cycler
7
Q
dNTP
A
Nucleotides that contain deoxyribose sugar
8
Q
ddNTP
A
Nucleotides that contain dideoxyribose sugar
