PCR Flashcards
1
Q
What are the steps of PCR?
A
Denature, annealing, transcription
2
Q
What factors contribute to the denaturing phase of PCR?
A
Denature by the SEA
S –> SALT, ionic strength
E –> Elevated temperature (95C)
A –> Alkaline pH
3
Q
What temperature does primer annealing occur?
A
50-60C
4
Q
What temperature does extension occur in PCR?
A
72C
5
Q
Each cycle replicates how much DNA?
A
Doubles the amount of DNA
