DNA Flashcards
1
Q
What is a palidrome?
A
Same sequence on both DNA strands when read in either direction:
5’ GFTACC 3’
3’ CCATGG 5’
2
Q
How to calculate nucleic acid purity?
A
A260/A280
3
Q
How to calculate DNA concentration?
A
A260 x dil factor x 50
4
Q
How to calculate RNA concentration?
A
A260 x dil factor x 40
5
Q
What are the steps in isolating DNA from clinical specimens?
A
Some Lazy Dogs Can Play Right
Separate, Lyse, Denature, Contaminants, Precipitate, Resuspend
6
Q
What are the phases of Mitosis?
A
The Pro Met Ana at the Telephone to Introduce himself
Prophase Metaphase Anaphase Telophase Interphase
7
Q
Which one is in the nucleus: Introns or Exons?
A
Introns
Remain IN the nucleus