Electrophoresis Flashcards
1
Q
Separation of DNA/RNA electrophoresis is based on?
A
Size and charge
2
Q
What charge is DNA?
A
Negatively charged. Will migrate towards positive end (anode)
3
Q
How to remember factors that affect DNA movement through a gel?
A
PENS
P –> pore size of gel
E –> electric field strength
N –> negative DNA charge
S –> size of DNA
BIG DNA molecules –> migrate slow
Small DNA molecules –> migrate fast
