Pbio Enzyms Pt 2

Question 1

Factors that affect enzymes activity

Answer 1

Temperature
pH
Substrate concentration
Enzyme concentration

Question 2

Temperature at which the rate of enzyme catalyzed reaction is maximum

Answer 2

Optimum temperature

Question 3

Optimum temperature for human enzymes is

Answer 3

37c

Question 4

Increased temperature (high fever)

Answer 4

Leads to decreased enzyme activity

Question 5

Drastic changes in pH can result in

Answer 5

Denaturation of proteins

Question 6

PH at which enzyme has maximum activity

Answer 6

Optimum pH

Question 7

Most enzymes have optimal activity in pH range of

Answer 7

7.0-7.5

Question 8

Exception in pH

Answer 8

Digestive enzymes

Question 9

Optimum pH of Pepsin

Answer 9

2.0

Question 10

Optimum pH of Trypsin

Answer 10

8.0

Question 11

A constant enzyme concentration, the enzyme activity increases with increased substrate concentration

Answer 11

Substrate concentration

Question 12

Concentration at which it reaches its maximum rate and all of the active sites are full

Answer 12

Substrate saturation

Question 13

Number of substrate molecules converted to product per seconde per enzyme molecule under conditions of optimum temperature and pH

Answer 13

Turnover number

Question 14

At a constant substrate concentraion

Answer 14

Enzyme act increases with increse in enzyme concentration

Question 15

A microorganism that thrives in exterme environments, environments which humans and most other forms of life could not survive

Answer 15

Extremophile

Question 16

Optimal growth at pH levels of 2 and lower

Acidobacterium leptospirillum picrophilus and ferrosplasma

Answer 16

Acidophiles

Question 17

Optimal growth at pH levels of 9.0 or above

Bacillus pseudofiirmus, b halodurans

Answer 17

Alkaliphiles

Question 18

High salinity that exceeds .2M NaCl for growth

Answer 18

Halophiles

Question 19

A temp between 80c and 121 c needed to thrive

Answer 19

Hyperthermophiles

Question 20

High hydrostatic pressure needed for growth

Answer 20

Piezophiles

Question 21

Extremely dry conditions needed for growth

Answer 21

Xerophiles

Question 22

Temp of 15c or lower for growth

Answer 22

Cryophiles

Question 23

A substance that slows down or stops the normal catalytic function of an enzyme by binding to it

Answer 23

Enzyme inhibitor

Question 24

Compete with the substrate for the same active site

Answer 24

Competitive inhibitors

Question 25

Do not compete with the substrate for the same active site
Binds at a different location

Answer 25

Non competitive