Pathogenesis of periodontal diseases Flashcards
Gram positive vs gram negative bacteria
Gram positive: only one membrane and a thick peptidoglycan layer which when included in the gram stain method retains the stain in side the cell causing it to appear purple
Gram negative: two membranes with a thin cell wall between the two mems creating a space called the peroiplasm. And have an outer capsule of LPS. Does not retain the stain so appears pink under light microscope
Koch’s postulates
traditional view of infection. One organism –> one infection
Not seen as the case anymore
Periodontal progression
Health –> gingivitis (inflammation) –> moderate periodontal disease (pocket) –> advanced periodontal disease (bone loss)
Loe longitudinal studies
Gingivitis develops when plaque accumulates
Health restored when plaque removed
Germ-free animals
No bacteria, no disease
Germ-free plus bacteria –> disease (but not all animals equally)
Periodontitis-causing bacteria ‘transmissable’ (disease may or may not arise)
Layers of organisms in subgingival plaque
Firmly attached: deeper layer, predominantly gram+
Loosely attached: more superficial layer, gram- anaerobes and motile bacteria
Exist in close, mixed communities
Disease progression
Sporadic and often site specific
What changes a quiescent site to an active one?
Change in host - immune status, age, environmental factors (smoking etc) Change in microbial challenge -type of organisms -number of particular organisms -virulence of organism
Problems with sampling subgingival plaque
If tooth is posterior and difficult to access
Might sample supragingival plaque
Instruments to sample subgingival plaque
Use a curette or paper point
The idea of the (oral) microbiome
The microbiome affects the way that you live
In our bodies we carry around 1kg mass in bacteria (mainly in digestive tract)
Profound influence on our health
-GU health (females)
-GU health > IBD
-GI food processing > obesity
Establishing microbes in subgingival plaque
Methods:
- Culture up to late 90s
- Sequencing 16s rRNA PCR clone libraries/ hybridisation arrays
- Mass sequencing of 16s rRNA amplicons
Why do we use 16s rRNA sequencing to identify species?
16s rDNA very well conserved due to esssential function
Acts as molecular clock and species signature as evolves slowly in time
We sequence it to speciate bacteria (18s is human/ animal equivalent)
Firmicutes & streps
Omnipresent in samples, not contributing to disease
Bacteroidetes
Raised in disease
Anaerobic, Gram- bacilli