Pathogenesis of periodontal diseases Flashcards

1
Q

Gram positive vs gram negative bacteria

A

Gram positive: only one membrane and a thick peptidoglycan layer which when included in the gram stain method retains the stain in side the cell causing it to appear purple
Gram negative: two membranes with a thin cell wall between the two mems creating a space called the peroiplasm. And have an outer capsule of LPS. Does not retain the stain so appears pink under light microscope

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2
Q

Koch’s postulates

A

traditional view of infection. One organism –> one infection
Not seen as the case anymore

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3
Q

Periodontal progression

A

Health –> gingivitis (inflammation) –> moderate periodontal disease (pocket) –> advanced periodontal disease (bone loss)

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4
Q

Loe longitudinal studies

A

Gingivitis develops when plaque accumulates

Health restored when plaque removed

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5
Q

Germ-free animals

A

No bacteria, no disease
Germ-free plus bacteria –> disease (but not all animals equally)
Periodontitis-causing bacteria ‘transmissable’ (disease may or may not arise)

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6
Q

Layers of organisms in subgingival plaque

A

Firmly attached: deeper layer, predominantly gram+
Loosely attached: more superficial layer, gram- anaerobes and motile bacteria
Exist in close, mixed communities

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7
Q

Disease progression

A

Sporadic and often site specific

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8
Q

What changes a quiescent site to an active one?

A
Change in host - immune status, age, environmental factors (smoking etc)
Change in microbial challenge
-type of organisms
-number of particular organisms
-virulence of organism
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9
Q

Problems with sampling subgingival plaque

A

If tooth is posterior and difficult to access

Might sample supragingival plaque

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10
Q

Instruments to sample subgingival plaque

A

Use a curette or paper point

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11
Q

The idea of the (oral) microbiome

A

The microbiome affects the way that you live
In our bodies we carry around 1kg mass in bacteria (mainly in digestive tract)
Profound influence on our health
-GU health (females)
-GU health > IBD
-GI food processing > obesity

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12
Q

Establishing microbes in subgingival plaque

A

Methods:

  1. Culture up to late 90s
  2. Sequencing 16s rRNA PCR clone libraries/ hybridisation arrays
  3. Mass sequencing of 16s rRNA amplicons
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13
Q

Why do we use 16s rRNA sequencing to identify species?

A

16s rDNA very well conserved due to esssential function
Acts as molecular clock and species signature as evolves slowly in time
We sequence it to speciate bacteria (18s is human/ animal equivalent)

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14
Q

Firmicutes & streps

A

Omnipresent in samples, not contributing to disease

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15
Q

Bacteroidetes

A

Raised in disease

Anaerobic, Gram- bacilli

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16
Q

Unculturables

A

~500 species present

~50% can be cultured

17
Q

Evidence for specific microbial aetiology

A

High numbers of certain bacteria cultured from diseased sites
Can cause disease in certain animal models
Have demonstrable virulence factors

18
Q

Groups of organisms in periodontitis

A

Health: S. mitis, S. oralis
Disease: P. gingivalis, Ta. forsythia

19
Q

P. gingivalis

A

Black pigmented anaerobe
Lipopolysaccharide in outer membrane
Gram negative
Secretes gingipains - virulence factors (proteases which degrade protein)
Epithelial cell invasion - immune evasion and recolonisation after debridement (hides)
Surface proteins key to human-cell interaction

20
Q

Ta. forsythia

A

Anaerobe (not black)
Gram -ve
Unusual structure
Tooth-like S- layer: responsible for aspects of adhesion (glycosylated?)
Fibronectin binding protein
Protease/ toxins
Glycosidases give it competitive advantage

21
Q

Sheffield findings Tannerella

A

Sialic acid is key nutritional source for Tannerella
Acquisition of sialic acid requires cleavage from host proteins
-inhibition with Tamiflu stops growth. New treatment?

22
Q

T. denticola

A

Small, thin spiral shaped bacterium
Few known virulence factors
Difficult to grow and work with

23
Q

Groups of organisms are more important than single pathogens

A

Diff bacteria have diff virulence factors
Combination of these factors more likely to cause disease
Qualitative mixture of pathogens determines disease progression

24
Q

Dysbiosis

A

Move from health to disease

Healthy community –> environmental factors –> disease community

25
Q

Keystone hypothesis

A

P. gingivalis has been shown to shift whole pop. from non-pathogenic to pathogenic in mouse models even if only present in low numbers
Also noticed in obesity

26
Q

Imbalance in innate immunity

A

Can –> bone loss

27
Q

Mechanisms of tissue damage

A

Bacteria:

Evasion of host defences, induction of inflammation (cytokines) –> bone resorption, soft tissue damage

28
Q

Necrotising ulcerative gingivitis

A

Tissue damage
Depression of host defences by smoking and modification of response by stress
Possibly depression of peripheral blood supply to tips of papillae
Selection of specific bacteria by host-derived nutrients
Fuso-spirochaetal complex

29
Q

Polymicrobial infections

A

Caused by interactions between 2 or more organisms leading to disease
Sum of parts and their virulence factors cause disease, not one in isolation
Interaction with host defences often key