Path: HIV & Associated Inf Flashcards

1
Q

What are the features of acute retroviral syndrome?

A

3-6 wks after inf, resolves in 2-4 weeks
seeding of lymphoid organs, high virus replication
mono-like syndrome: rash, cervical lymphadenopathy, N/V

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2
Q

What laboratory testing is done for HIV?

A

serology:
screening - enzyme immunoassays for Abs and p24
confirmatory - western blot and immunofluorescence
differentiation assay - HIV-1 vs. HIV-2
molecular assays

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3
Q

What are the basics of enzyme immunoassays (EIA) for testing in HIV?

A

Abs/Ags appear 2-8 wks following inf

screening test, positives followed by confirmatory or differentiation assay

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4
Q

What are the basics of immunofluorescence assays for testing in HIV?

A

HIV inf cells fixed to slide, add pt serum and incubate
wash and add anti-human Ab w fluorescent tag
look for fluorescing cells
only detects HIV-1, first generation

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5
Q

What are the basics of the HIV-1/HIV-2 differentiation assay?

A

EIA that detects Abs, second generation

cheaper, quicker and easier than WB

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6
Q

What are the molecular assays used for testing in HIV?

A

PCR: qualitative (diagnosis but not first line test, screening for blood products), quantitative (predict viral load and progression of dz, monitor response to Tx)

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7
Q

What are the basics of the HIV genome?

A

gag region - forms p17 and p24
pol region - encodes protease, integrase and reverse transcriptase
env gene - encodes gp120 and gp41

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8
Q

What are the basics of the WB used for testing in HIV?

A

technically challenging
detects Abs to gp120, gp41, p24
first generation - only detects IgG - can lag up to 3 wks and produce false negatives

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9
Q

What is the basic testing algorithm for HIV?

A

start with fourth generation assay
confirm + w rapid IgG immunoassay (2nd) that differentiations HIV-1 from HIV-2
negative or indeterminate tested for HIV-1 RNA
negative, further eval needed

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10
Q

What is the testing algorithm for HIV at Parkland?

A

3rd gen test as screen
+ confirmed w IFA
negative/indeterminate IFA send to reference lab for WB or HIV-2

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11
Q

What are the basics of CMV inf in HIV?

A

primary asymptomatic or mono-like, latency in WBCs, reactivation in immunocompromised
retinitis, colitis, esophagitis, pneumonitis, CNS
dx by biopsy or quantitative PCR on blood, owl’s eye nuclear inclusions

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12
Q

What are the features of the CNS dz seen in pts w HIV?

A

aseptic meningitis w/i 1-2 wks of seroconversion - CSF has lymphocytes, protein, normal glucose
encephalopathy

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13
Q

Positive stain by mucicarmine indicates what pathogen?

A

crypto

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14
Q

What are the features of PML in pts with HIV?

A

JC polyoma virus (DNA)
infects oligodendroglial cells
multifocal demyelination, global encephalopathy, variable focal neurologic deficits
dx by imaging, PCR on CSF, biopsy, white matter has granular appearance grossly

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15
Q

What syndromes can CMV cause in HIV?

A

encephalitis, ventriculitis/choroid plexus, radiculoneuritis (lower cord and roots)

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16
Q

What are the basics of Kaposi sarcoma in HIV?

A

vascular tumor, often multifocal
spindle cell proliferation
endothelial and smooth muscle muarkers
more common w sexually transmitted HIV

17
Q

What are the features of non-Hodgkin lymphomas in HIV?

A

systemic: nodes and viscera, half are EBV
primary CNS: virtually all EBV
body cavity based
EBV is polyclonal B cell mitogen - polyclonal activation followed by emergence of monoclonal pop

18
Q

What is oral hairy leukoplakia?

A

EBV drive squamous proliferation

not a malignancy, hyperplastic process