Pasturella and Similar Organisms Flashcards
List general characteristics of Pasturellaceae
- Sm., GNB
- Non-motile
- Oxidase pos
- Ferms glucose
- Most don’t grow on MAC
Where is Pasturella found?
- Domestic/wild animals
- Not NF
How is it transmitted?
- Close anmial contact
- Opportunistic )from bites)
Virulence factors of P. multocida
- Lipopolysaccharides
- Cytotoxins
- 6 serotypes of capsule
- Surface adhesions
- Iron-acquisition proteins
Pathogenesis and spectrum of disease for P. Multocida
- Respiratory disease + sytemic disease (endocarditis, septicemia)
- Liver cirrhosis
- Soft tissue infection
Gram stain; P. spp.
Usually short, straight bacilli w/ frequent bipolar staining
- P. aerogenes; coccobacilli
- P. bettyae; usually thin
Gram of Mannhemia haemolytica
Sm. bacillus/coccobacillus
Gram of Suttonella indologenes
Broad bacillus, variable length
Culture of Pasturella spp.
- TSA supplemented w/ 5% sheep blood
- CHOC
- Most won’t grow on MAC (except: some P.bettyae + P.pneumotropica
- Grows well on BC broth + nutrient broths
Culture from animal bites:
- MAC = NG
- Gram = GNCB (indicates P. spp)
How do you differentiate Pasturells spp. from Haemophilus spp.?
Via CO2 independence and growth on BA
ID of Pasturella multocida
- Gram: Sm, straigh GNB Tests: - Catalase pos - Oxidase pos - Ferments gluc - Reduces nitrate to nitrite - Indole pos - Ornithine pos - Urea neg
Definitive ID of Pasturella multocida
- Will ID w/ API 20e
- Molecular technique
- MALDI-TOF 9
- May need to be sent to reference lab
How do you differentiate Mannhemica haemolytica fom p. spp.?
Mann. can produce indole and ferment mannose.
How do you differentiate Suttonella indologenes from P. spp.?
Sutt. has neg nitrate test
