Part II: Fundamentals Flashcards
How do we grade agglutination reactions?
- Size of the RBC agglutinate
- Color of supernatant fluid (SF)
% Ag: 100%
Score: 12
Description of appearance:
- One solid clump, no free
cells, clear supernatant
4+
% Ag: 75%
Score: 10
Description of appearance:
- Several large clumps, clear supernatant
3+
% Ag: 50%
Score: 8
Description of appearance:
- Many medium-sized clumps, cloudy red supernatant
2+
% Ag: 25%
Score: 5
Description of appearance:
- numerous small clumps, cloudy red supernatant
1+
% Ag: <25%
Score: 3
Description of appearance:
- numerous very small clumps, ,easily dispersed cloudy red supernatant
+/-
% Ag: <25%
Score: 1
Description of appearance:
- appears negative macroscopically, agglutination visible microscopically
+
% Ag: 0
Score: 0
Description of appearance:
- Negative, no agglutination
seen macroscopically
0
Complete hemolysis, no intact RBCs remaining
H
Partial hemolysis, some RBC sill intact; Hemolysis is visible
PH
Mixed field- mixtures of agglutinated and unagglutinated cells
● Cannot be determined
macroscopically
● Confirm using different
microscopic fields
mf (mixed field)
Normally the red blood cell surface transforms
into a net _____ because of
____ also called NANA.
NEGATIVE CHARGE ; SIALIC acid
To decrease the zeta potential add
POTENTIATOR
Antibody-like substances or agents obtained or
derived from plant sources specifically seed
extracts that could detect specific antigen
LECTINS
LECTINS
Anti-A1
Dolichos biflorus
