Para Lab 6 Notes

Question 1

causative agent of hepatozoonosis

Answer 1

hepatozoon canis

Question 2

is hepatozoonosis heteroxenous or homoxenous?

Answer 2

obligate heteroxrnous

Question 3

what is the biological vector of hepatozoon canis

Answer 3

Rhipicephalus sanguineus

Question 4

symptoms / clinicial signs of hepatozoonosis

Answer 4

frequently asymptomatic

lethargy, fever, anorexia, anaemia, swelling of LN, lachrymation, nasal discharge.

Question 5

in what cells can we find hepatozoons

Answer 5

neutrophil granulocytes

Question 6

in what form can we find hepatozoon in neutrophil granulocytes

Answer 6

gamont

Question 7

what type of ssample is required for a parasitological diagnosis of hepatozoon?

Answer 7

blood smear

Question 8

what size are the gamonts of hepatozoon in neutrophil grnaulocytes?

Answer 8

10 - 12 um elliptical

Question 9

what size is hte schizont of a hepatozoon

Answer 9

50 - 100um

Question 10

what type of wall and locaiton of nuclei in the schizont of a hepatozoon?

Answer 10

unilaminar wall
nuclei on the peripehery

Question 11

babesia species found in equine

Answer 11

babesia caballi

Question 12

babesia species found in cattle

Answer 12

b divergens - zoonotic
b bovis
b bigemina
b major

Question 13

babesia species found in canine

Answer 13

b canis
b vogeli
b gibsoni

Question 14

what is the most zoonotic species of babesia

Answer 14

b divergens

Question 15

in what species can abortion occur in babesiosis infection

Answer 15

cattle

Question 16

what species does hemoglobinuria not occur in for babesiosis infection

Answer 16

does not occur in horses in hte case of b caballi

Question 17

what symptoms occur in digs in hte case of babesiosis infection

Answer 17

keratitis
iridocyclitis
nervous signs

Question 18

what are the signs of chronic babesiosi infection

Answer 18

intermittent fever for several months
gradual emaciaiton
anaemia

Question 19

babesiosis form found in RBCs

Answer 19

piroplasms

Question 20

what staining is used to detect babesia piroplasm in RBCs

Answer 20

giemsa staining
red nucleus, blue cytoplasm.

Question 21

typical appearance of ‘small babesia’

Answer 21

b divergens
<3um
typically appear as a paired near edge of the cell

Question 22

typical appearance of ‘large babesia’

Answer 22

b . bigemina
3 - 5um
paired at acute angle in RBCs

Question 23

shape of babesia species

Answer 23

pyriform
round
elongated
cigar shaoed

Question 24

necropsy findings of babesiosis infection

Answer 24

sub-epicardial and sub-endocardial haemorrhages
red abd brownred urine in bladder
catarrh and petechiae in GI mucosa

Question 25

theileria soecies tyhat infects horses

Answer 25

T. equi

Question 26

theileria species that infects cattle

Answer 26

t annulata

Question 27

clinical signs of theileria infection

Answer 27

digestive problems - rumination abandoned
diarrhoea with mucos and blood
swelling of LN
petechial haemorrhages on vulva and under tongue

Question 28

what for of theileria are found in LN

Answer 28

schizonts of theileria
use Giemsa staining

Question 29

what form of theileria is found in lymphocytes

Answer 29

KOCHs bodies
12um
in cytoplasm of lymphocytes

Question 30

what form of theileria can be found in RBCs

Answer 30

merozoites can be found in RBCs

Question 31

what is the size and shape of theileria merozoites

Answer 31

0.5 - 2.7um
rod shaped
blue

also have round, oval, ring-shaped forms

Question 32

what are the necropsy findings related to theileria infection

Answer 32

haemorrhages in serous and mucous membranes
swelling of LN and spleen
ulcers in abomasum and intestine
pulmonary oedema

Question 33

in the case of theileria infection where can haemorrhages be found

Answer 33

serous and mm

Question 34

in the case of a theileria infeciton where can ulcers be found

Answer 34

abomasum and intestine

Question 35

in what species is encephalozoonosis mainly found

Answer 35

MAMMALS
encephalitozoon cuniculi

Question 36

what species of mammal can we mainly find encephalitozoonosis infection

Answer 36

rabbit

Question 37

what symptoms of encephalitozoonosis can occur in rabbits

Answer 37

torticollis
wry neck
paralysis
seizures

Question 38

what symptoms of encephalitozoonosis can occur in fox and dog

Answer 38

keratitis
night blindness

Question 39

size of shape of encephalitozoon spores

Answer 39

1.5 - 2.5um elliptic or oval

Question 40

where can we find encephalitozoon spores in rabbits

Answer 40

urine

Question 41

if spores of encephalitozoonosis are expected what staining method can we sue

Answer 41

indian ink rxn
IIR

ink particles adhere to spores on slide

Question 42

if antibodies are expected to be found in serum of animals infected with encephalitozoonosis what test can be used

Answer 42

oil immersion method

Question 43

what can be found on the surface of kidneys of dogs and rabbits infected with encephalitozoonosis

Answer 43

2 - 4mm greyish dents

Question 44

in the case of encephalitozoonosis infection what histological findings can be found in the kidneys

Answer 44

chronic interstitial nephritis

Question 45

in the case of encephalitozoonosis infection what histological findings can be found in the brian

Answer 45

multifocal granulomatous encephalitis

perivascular infiltration by lymphocytes
necrotic foci surrounded by macrophages

Question 46

what staining can be used to detect encephalitozoonosis

Answer 46

PAS
Gr +
ZN