Oxidative Phosphorylation

Question 1

Where about in mitochondria

Answer 1

Cristae

Question 2

What is the function of 
Ox P (4)

Answer 2

Produce energy

Oxidise NADH and FADH

Restore cofactors

Produce water

Question 3

What are the substrates

Answer 3

NADH and FADH

Protons - to create gradient

Oxygen

ATPase synthase

Question 4

Product

Answer 4

ATP

NAD and FAD

Water

Question 5

What is the first step of Ox P

Answer 5

Electron chain transport

Question 6

What are the steps of electron chain transport (4)

Answer 6

Series of proteins in membrane

Cofactors oxidised

Protons moved to Cristae

Proton gradient forms

Question 7

What are the stages of step 2 of Ox P

Answer 7

Proton gradient moves H back to matrix

Energy of this

ADP to ATP

Question 8

What enzyme is needed to convert ADP to ATP

Answer 8

ATPase synthase

Question 9

How many complexes are there in electron transport

Answer 9

4

Question 10

Which complex does not have proton pump

Answer 10

2

Question 11

Complex 1(3)

Answer 11

Oxidised NADH

Passes electron to ubiquenone

Proton pump

Question 12

Complex 2 (2)

Answer 12

Oxidises FADH

Passes e to ubiquenone

Question 13

Complex 3 (3)

Answer 13

Oxidises ubiquenone

Takes electrons reduces cytochrome C

Proton pump

Question 14

Complex 4 (3)

Answer 14

Reduces oxygen to water

Electrons go to oxygen

Proton pump

Question 15

What does transfer of protons do

Answer 15

Creates gradient

Question 16

Location

Answer 16

Mitochondria

Brown adipose (neonate)

Question 17

What are the two regions of Fo F1 ATPsynthase

Answer 17

Fo
- transmemvrane proton channel

F1

• central gamma unit
• surrounded by 3 alpha and 3 beta units

ADP binds to beta and spins
Energy creates ATP

Question 18

Control of Ox P

Answer 18

Respiratory
- limited by oxygen

Level of ADP

Question 19

What happens to proton channel when low ADP

Answer 19

Closes