Krebs Flashcards
What sources of building blocks come from krebs
Glucose Cholesterol Porphyrin AA Nucleotide bases
What is the type of reactions between substrates
OAA and acetyl CoA
To
Citrate
= condensation
To
OAA
= decarboxylated and oxidised
What is the overall function of krebs
To harvest high energy electrons
Yield
2 CO2
1 GTP
3 NADH
1 FADH
= 8 e to OxP
3 H+
Site
Matrix of mitochondria
What enzymes are involved in converting
Pyruvate to acetyl CoA
OAA to citrate
Pyruvate dehydrogenase
Citrate synthase
What is added produced and converted
Pyruvate to acetyl CoA
CoA added
Co2 produced
NAD to NADH
Where does mitochondria originate
Bacteria
Substrates for krebs (4)
Acetyl CoA enzyme
Carbon sources
Oxidising factors
- NAD and FAD
GDP & PI
- GTP
Acetyl CoA derives from (4)
Pyruvate
Ketone bodies
Ketogenic aa
FA breakdown - mitochondria
Other carbon sources
a -ketogluterate
Succinyl CoA
Control 1
Up regulate
(Exercise heat disease)
Down regulate
(Rest hibernate recovery)
Whole body (Endocrine CNS environment FF)
Cellular
(Ratio ADP:ATP NADH :NAD)
2nd level of control
Pyruvate dehydrogenase
Inhibited by product - acetyl CoA & NADH
Stimulated by substrate - Pyruvate & NAD d
Insulin stimulates
What are 2 dates of carbons from TCA
Oxidised - CO2 & energy
Incoperated into fats
Why is Pyruvate dehydrogenase complex so tightly regulated
Can’t be reversed
