Oxidative Phosphorylation

Question 1

Where it occur

Answer 1

Cristae of mitochondria

Question 2

Process of Oxidative Phosphorylation

Answer 2

Reduced NAD/FAD oxidised to release H atoms which split into protons and electrons.
Electrons transferred down the ETC loosing energy at every step via redox reactions
Energy used to actively transport protons from the matrix to the intermembrane space.
Protons diffuse down electrochemical gradient via ATP synthase back into the matrix
This releases energy to combine ADP+Pi to form ATP.

At the end of the ETC is oxygen which is the terminal electron acceptor- protons, electrons and O2 combine to form water

Question 3

Why is Oxygen needed for the production of ATP on the cristae of the mitochondrion

Answer 3

Oxygen= terminal electron acceptor for electrons passing along the ETC. ETC releases energy for the formation of ATP.
No O2 to accept them= electrons cannot be passed along the ETC.
So the kreb cycle and Link reaction also stop because reduced NAD and FAD no produce

Question 4

what are the other respiratory substrates

Answer 4

Lipids and amino acids are broken down and enters into the Krebs Cycle. For example:
-Fatty acids from the hydrolysis of lipids are converted to Acetyl CoA.
-Amino acids from the hydrolysis of proteins are converted to intermediates in Krebs Cycle.

Question 5

How to calculate RQ (Respiratory Quotient)

Answer 5

RQ= Volume of CO2 given out/ Volume of O2 taken in

Question 6

Glucose and its RQ value

Answer 6

Anaerobic respiration because glucose broken down.
RQ value >1

Question 7

RQ value of carbohydrates, Proteins and Lipids

Answer 7

Carbs= 1.0
proteins= 0.8-0.9
Lipid = 0.7

Type of respiration is Aerobic Respiration