Overview of Histopathologic Techniques

Question 1

First and most important step in histopathology

Answer 1

Numbering

*Specimen no.

Question 2

Specimen details must be entered in _

Answer 2

Log book

Question 3

Format for specimen numbering
S-
A-
C-

Answer 3

S- surgical
A- autopsy
C- cytology

• Year is also added after SAC
  Ex. S99-0345

Question 4

T/F:
There should be a separate logbook for every type of specimen
(surgical, autopsy, cytology)

Answer 4

True

Question 5

Used for labeling the slide and for writing the gross description (stated by the pathologist)

Answer 5

Pencil

* It will not be washed out

Question 6

Specimen size for processing

Answer 6

3x2 cm and 3-5mm thick

Question 7

This can be added for too small tissues when processing

Answer 7

1% eosin

* Prevents loss of the tissue (since its too small)

Question 8

Size of tissue for electron microscopy

Answer 8

1mm^3

Question 9

Dimension of tissue casette

Answer 9

2.5x4cm (5mm depth)

Question 10

Process of preserving cells and tissue constituents in a condition identical to that existing during life

Answer 10

Fixation

Question 11

Two important goals of fixation

Answer 11

1. Preserve the morphological and chemical integrity of the cell
2. Harden and preserve tissue for further handling

Question 12

Most crucial step in Histopathology

Answer 12

Fixation