Fresh Tissue Preparation

Question 1

• Tissue is immersed in a watch glass with NSS
• Dissected or separated using a dissecting needle/applicator stick
• Stained using methylene blue
• Examined under phase contrast/ brightfield

Answer 1

Teasing / Dissociation

Question 2

• Small tissues (1mm or less) placed in a slide
• Tissues compressed with another slide/ coverslip
• Supravital stains are added

Answer 2

Squash preparation

- 1mm / less

Question 3

Cellular materials are spread lightly over a slide

Answer 3

Smear preparation

Question 4

Material is added using an applicator stick/loop in a zigzag fashion

Answer 4

Streaking

Question 5

Material is placed on a clean slide and spread into a moderately thick film through teasing with an applicator stick

Answer 5

Spreading

> Maintains cellular relationships

Question 6

For thick secretions, uses 2 slides

Answer 6

Pull Apart

Question 7

Uses freshly cut piece of tissue brought into contact and placed on the surface of a clean slide

Answer 7

Touch preparation / Impression smear

Question 8

Slide used for touch preparation/ Impression smear

Answer 8

Sterile & polished edges

Question 9

For rapid diagnosis (benign/malignant) utilized in the OR intraoperatively (done during operation)

Answer 9

Rushed Frozen Section

Question 10

Time for Rushed Frozen Section

Answer 10

5-15 min.

Question 11

Apparatus for Rush Frozen Section

Answer 11

Freezing microtome

Cryostat/cold microtome