Fixation Flashcards
1
Q
Two mechanisms of action of fixatives
A
- Additive
2. Non-additive
2
Q
Mechanism of fixation wherein the fixative becomes a part of the tissue by the formation of cross-links
A
Additive fixation (Formalin, Osmium tetroxide)
3
Q
Mechanism of fixation wherein fixative is not incorporated into the tissue, but stabilized it by the removal of water present
A
Non-additive fixation (Alcoholic fixatives)
4
Q
pH of Fixation
A
6-8 pH
5
Q
Temperature for fixation in:
a. Traditionally
b. Autotech
c. EM and Histochem
d. Rapid Fixation
e. For tissues w/ TB
A
a. Traditionally - Room temp
b. Autotech - 0-4C
c. EM and Histochem - 0-4C
d. Rapid Fixation - 60C
e. For tissues w/ TB- 100C
