overview of hematology Flashcards
what are the three types of blood and also the solid portion of the blood?
Red Blood Cells (RBCs) / erythrocytes
White Blood Cells (WBCs) / leukocytes
Platelets (PLTs) / thrombocytes
study of blood cells using staining,
counting, analyzing and recording of appearance,
genotype and phenotype of each cell.
Hematology
Anucleated, biconcave, discoid cells filled with protein
(hemoglobin)
Red blood cells
Transports oxygen and carbon dioxide
Red blood cells
loss of oxygen-carrying capacity of blood
▪ reduced RBC count or reduced RBC hemoglobin
concentration
Anemia
▪ increased RBC count
▪ increased circulating RBC mass that leads to
hyperviscosity.
Polycythemia
_______ matches the osmolality of RBC
Normal saline
what is the dilution of RBC
1:200
a glass pipette designed for dilution
thoma pipette
it is called a glass counting chamber
hemacytometer
RBCs are assayed for?
Hemoglobin concentration and Hematocrit
HGB measurement relies on what solution?
weak solution - potassium cyanide and potassium ferricyanide
potassium cyanide and potassium ferricyanide is called?
Drabkin reagent
it is a mixture of whole blood and drabkin reagent
cyanmethemoglobin
what is the absorbance of cyanmethemoglobin in spectrometer?
540 nm wavelength
alternative for ionic surfactant?
sodium lauryl sulfate
ratio of the volume of packed RBCs to the
volume of whole blood.
Hematocrit
also called “packed cell volume”
hematocrit
light-colored layer between RBCs and
plasma which contains WBCs and platelets
buffy coat
volume measurement
recorded in femtoliters (fL). RBC diameter on Wrightstained blood film
mean cell volume
grams
per deciliter (g/dL). RBC staining intensity and central
pallor
mean cell hemoglobin concentration
variation in diameter or size?
anisocytosis
Indicate the ability of bone marrow to increase RBC
production
polychromatic erythrocytes
used to differentiate and count young RBCs
methylene blue
- present as protection against infection and injury.
- produce in bone marrow or lymphoid tissue.
white blood cells
what is the dilution of WBC
1:20