Overview Flashcards
Chromosome number of prokaryotes
One
With membrane-bound organelles
Eukaryotes
Plants and algae component of cell wall
Cellulose
Cell wall of archaebacteria is composed of
Pseudopeptidoglycan
Motility structures of bacteria
Flagella
Cell wall of fungi contains
Chitin
Microorganisms without cell wall but with flexible membranes
Protozoa
Helminths
Obligate intracellular but acellular parasites of plants with naked RNA; not medically important
Viroids
Those with true nuclear membrane
Eukaryotes
Gram +/- bacteria: has teichoic acids
Gram positive
Gram +/- bacteria: periplasmic space
Gram negative
Gram +/- bacteria: has thicker peptidoglycan layer(s)
Gram positive
Steps and reagent for gram staining
1: Primary stain - Crystal violet
2: Mordant - Iodine
3: Decolorize - Acetone/ alcohol
4: Counterstain - Safranin
All bacteria have cell walls made out of peptidoglycan except
Mycoplasma pneumoniae
All gram+ bacteria have no endotoxin except
Listeria monocytogenes
All bacteria capsules are composed of polysaccharide except
Bacillus anthracis
All exotoxins are heat-labile except
Staphylococcal enterotoxin
Examples of obligate aerobes
Leptospira, Mycobacterium, Mycoplasma pneumoniae, Pseudomonas, Nocardia, Bacillus cereus, Neisseria, Bordetella, Legionella and Brucella
Examples of microaerophiles
Campylobacter, Helicobacter, Streptococcus, Spirochetes (Borrelia, Treponema)
Examples of facultative anaerobes
Staphylococcus, Bacillus anthracis, Corynebacterium, Listeria, Mycoplasma (non-pneumoniae)
Examples of Aerotolerant anaerobes
Propionibacterium, Lactobacillus
Examples of obligate anaerobes
ABC - Actinimyces, Bacteroides, Clostridium
Process when DNA is transferred from one bacterium to another
Conjugation
Prokaryotes
Process when DNA transferred by a virus from one cell to another
Transduction
In prokaryotes
Process when purified DNA is taken up by a cell
Transformation
In prokaryotes and eukaryotes
Cycles of bacterial transfuction (2)
Lytic cycle and Lysogenic cycle
Agent in the Griffith’s experiment
Streptococcus pneumoniae
mouse
Exo vs endotoxins: genes located in the plasmid or bacteriophage
Exotoxin
Exo vs endotoxins: low toxicity and antigenicity
Endotoxins
Exo vs endotoxins: meningococcemia
Endotoxin
Exo vs endotoxins: usually destroyed rapidly at 60C (except in Staph)
Exotoxin
Endotoxins are stable for 1 hour at which temperature?
100C