Overview 2 Flashcards
Loss of cells occurs where causing PD
Subsantia nigra pars compacta
What forms in the brain in a patient with PD?
Lewy bodies
What are Lewy bodies composed of?
Protein alpha-synuclein
What cells are lost in PD?
Dopaminergic cells
Where are dopaminergic cells lost in a patient with PD?
Substantia nigra pars compacta
What are the three main features/presentations of PD?
Resting tremor
Bradykinesia
Rigidity
Gradual development of what condition develops with PD?
Microphagia
Give five non-motor presentations of PD
Olfactory dysfunction Depression Dementia Sleep disturbance Autonomic disturbance
Non-motor presentations of PD can occur how much earlier than the motor symptoms?
Up to 12-15 years
Loss of dopaminergic cells in PD causes an imbalance in which two pathways?
Direct and indirect pathways of basal ganglia
What is meant by a score of 100% in the Schwab and England Activities of Daily living?
Patient is completely independent
How can the brain be imaged for dopaminergic cells in PD?
Dopamine transporter imaging
PD more common in males or females?
Males
MPTP has what effect on PD?
Can result in very advanced PD
Why can MPTP cause advanced PD?
Transformed into MPP+ which is neurotoxic to dopaminergic neurones
MPTP causes dysfunction of which organelle?
Mitochondria
What is the main enzyme causing oxidative stress in PD?
MAOb
Which of the dopaminergic pathways of the CNS is involved in PD?
Nigrostriatal pathway
Which of the dopaminergic pathways of the CNS is involved in PD?
Nigrostriatal pathway
What are the two groups of dopamine receptors?
D1
D2
D1 dopamine receptor groups consists of which dopamine receptors?
D1 and D5
D2 dopamine receptor groups consists of which dopamine receptors?
D2, D3, D4
Stages of metabolism of dopamine?
Dopamine to DOPAC to homovanillic acid
What is the role of MAO in dopamine metabolism?
Dopamine to DOPAC via MAO
What is the role of COMT in dopamine metabolism?
DOPAC to homovanillic acid via COMT
What type of receptor is the dopamine receptor?
G-protein coupled receptors
L-dopa can be used to treat dopamine because?
Pre-cursor - will be converted into dopamine
L-dopa should be given to a patient along with what?
Peripherally acting DOPA decarboxylase inhibitor
Name two other drug groups used for the treatment of PD? x2
MAOb inhibitors
Anticholinergic compounds
COMT inhibitors
Why are anticholinergic compounds used to treat PD?
Dopamine loss leads to hyperactivity of cholinergic cells
What is the role of amantadine in PD treatment? x2
Inhibits dopamine reuptake and increases dopamine release
What is apomorphine?
Dopamien agonist used as infusion for major motor fluctuations
What is rotigotine?
Dopamine agonist - transdermal patch
What is carbidopa?
Peripherally acting DOPA decarboxylase inhibitor
What is ropinerole?
Dopaminergic agonist
What is rasagiline?
MAOb inhibitor
What is benserazide?
Peripherally acting DOPA decarboxylase inhibitor
What is entacapone?
COMT inhibitor
What is tolcapone?
COMT inhibitor
What is tolcapone?
COMT inhibitor
Give 5 non-motor effects of L-DOPA
Nausea/vomiting Postural hypertension Psychosis Impulse-control disorders Excessive day-time sleepiness
Give four motor complications of L-dopa therapy
On-off effect
Wearing off
Dyskinesia/dystonia
Three types of therapy that should be offered to patients with PD?
Physiotherapy
Speech and language therapy
Occupational therapy
What is Huntington’s chorea?
Involuntary jerky movements
Cause of Huntington’s chorea?
Mutation on huntington protein of chromosome 4
Mutation involved with Huntington’s chorea is?
Abnormal number of repeats of glutamine (CAG)
Inheritance of Huntington’s chorea is?
Autosomal dominant
Four pathological changes in the brain seen in Huntington’s chorea?
Cortical atrophy
Striatal degneration
Loss of medium spiny neurones
Intranuclear inclusions of huntington
Main drug used to treat Huntington’s chorea?
Tetrabenazine
Main drug used to treat Huntington’s chorea?
Tetrabenazine
Two types of stroke are?
Ischaemic
Haemorrhagic
Main cause of intercerebral bleed is?
Hypertension
Four secondary causes of intercerberal haemorrhages?
Trauma
Tumour
Venous thrombosis
Drug usage
Four complications of ICH?
Local damage
Local mass effect/herniation
Raised ICP
Hydrocephalus
Three medical conditions that are risk factors for stroke are?
Hypertension
Hypercholesterolaemia
Diabetes
Cardiac condition that is a risk factor for stroke is?
AF
Deficit occurring from left hemisphere stroke is?
Reading and writing deficits
Prognosis for stroke?
1/3 - do well
1/3 - die
1/3 - poor prognosis
Prognosis for stroke?
1/3 - do well
1/3 - die
1/3 - poor prognosis
Three common symptom of MS?
Sensory/motor problems - numbness of the limbs/tingling sensation
Vision problems - blurriness/loss/diplopia
Progressive motor deficits
Three uncommon symptoms of MS
Bladder dysfunction
Heat intolerance
Dementia
What is MS?
Demyelinating, degenerative and inflammatory condition
What imaging is used for MS type conditions?
MRI
MRI signs of MS? x2
Lesion in corpus callosum
Areas of inflammation and brain atrophy
Non-imaging investigation used for MS?
Lumbar puncture
Three stages of MS disease progression?
Relapsing-remitting
Primary progressive
Secondary progressive
Lumbar puncture for MS - looking for what?
Presence of neurofilaments - oligoclonal bands
Four diagnostic criteria for MS?
Neurological deficits
Dissemination in space
Dissemination in time - damage occurred at two points in time
Exclusion of other causes
How many areas of the CNS must be damaged to diagnose MS?
At least two separate areas
Life expectancy of those with MS is reduced by what amount?
10-15 years
Loss of which cells occur in MS?
Oligodendrocytes
Loss of which cells occur in MS?
Oligodendrocytes
Oligodendrocytes produce what?
Myelin sheath
Four features of an MS lesion?
Astrocytic scar
Demyelination
Activated microglia
Oligodendrocyte loss
Cells involved in the demyelination of MS are?
Macrophages
Pathological hallmark of an MS lesion is?
Demyelinated axons
Infiltration of what cells occurs in MS?
T cells
T cells involved in MS are known as?
Autoreactive lymphocytes
T cells can enter brain in MS because?
BBB is damaged
Weighting of MRI for MS diagnosis?
T1
Differential diagnosis for MS? x4
Cerebrovascular disease
Syphilis
B12 deficiency
Lyme disease
Typical age of diagnosis of MS?
20s/30s
What comes first in MS - inflammation or degeneration?
We do not know
Relation of smoking to MS?
Smoking can cause MS
Four stages of MS treatment
Anti-inflammatory
Neuroprotection
Remyelination
Neuro-restoration
First line teatment for MS? x2?
IFNb
Natalizumab
Second line treatment for MS? x3
Fingolimod
Natalizumab
Alemtuzumab
Anatomical location of lumbar puncture?
Between L3 and L4 into subarachnoid space
Anatomical location of lumbar puncture?
Between L3 and L4 into subarachnoid space
Four physiological stages in the mechanism of pain?
Transduction
Transmission
Perception
Modulation
Transduction of pain involves what?
Noxious stimuli into electrical activity at sensory nerve endings
Endings of nociceptors are free or capsulated?
Free
Nociceptive channel that opens in response to heat thermal stimuli is?
TRPV1
Nociceptive channel that opens in response to cold thermal stimuli is?
TRPM8
Noxious fibres lost in diabetic neuropathy are?
C fibres
C fibres/a-delta fibres - which are myelinated?
A-delta
C fibres/a-delta fibres - which are fast conducting?
A-delta
C fibres/a-delta fibres - which is first and which is second pain?
A-delta - first, immediate pain
C - second, emotional pain
Resulting action from activation of a-delta fibres is?
Reflex withdrawal
Two classes of C fibres are?
Peptidergic c-fibres
Peptide-poor c fibres
Peptidergic C fibres release what? x2
Substance P
CGRP
Receptor of peptide poor c fibres is?
P2X3 - peptide poor C fibres
C fibre classes - which involves ATP receptors?
Peptide poor
A delta fibres project to which laminae?
I and V
C fibres project to which laminae?
I and II
What projects to lamina I?
All nociceptors
Gene responsible for the development of nociceptors is?
trkA
A-beta fibres enter/innervate which lamina?
V
What are projection neurones?
Second order neurones
What are VPL and VPM?
Nuclei:
Ventral posterior lateral
Ventral posterior medial
VPL and VPM project to where?
Primary somatosensory cortex
A-delta fibres mainly run into which spinal tract?
Anterior spinothalamic
Function of anterior spinothalamic tract is?
Regulates immediate need to withdraw arm from pain
A-delta fibres innervate which nuclei? x4
VPL
VPM
VPI (inferior)
CL (central lateral)
C-fibres mainly run into which spinal tract?
Lateral spinothalamic tract
C-fibres innervate which nucleus? x2
Posterior thalamus
Mediodorsal nucleus
Function of the lateral spinothalamic tract?
Regulation of punishing aspects of pain - prevent repetition
Lateral spinothalamic tract projects to where?
Anterior cingulate cortex (limbic system)
Rostral insular cortex
Anterior spinothalamic tract projects to where?
Primary and secondary somatosensory cortex
Lateral spinothalamic associated with which pain pathway?
Periaqueductal grey PAG
PAG pathway located where?
Midbrain
Lateral spinothalamic projects to which three specific areas?
PAG
Reticular formation
Parabrachial nucleus
Four signs of inflammation?
Calor - heat
Rubor - redness
Dolor - pain
Tumor - swelling
Inflammation results in what type of sensitisation?
Peripheral sensitiation
Three hallmarks of sensitisation?
Hyperalgesia
Allodynia
Spontaneous pain
Hyperalgesia is?
Abnormally heightened sensitivity to pain
Allodynia is?
Sensation of pain from non-noxious stimuli
Two features of peripheral sensitisation?
Reduced activation threshold
Increased responsiveness
NaV1.8 is?
Sodium channel
NaV1.9 is?
Sodium channel
Arachidonic acid released via what enzyme?
Phospholipase A2
Arachidonic acid converted to what?
Prostaglandins
Arachidonic acid converted to prostaglandins via what enzymes?
COX1
COX2
COX1 present what?
Normally present in all tissues at low levels
COX2 present when?
COX-2 induced during inflammation
Prostaglandins sensitise which pain fibres?
C-fibres
Prostaglandins are targeted by which pain relieving drug specifically?
NSAIDs
Central sensitisation caused by?
Prolonged nociceptive input
Central sensitisation results in what?
Modified response - low level inputs produce repsonse
Neurotransmitter involved in central sensitisation development?
Glutamate
Neuropathic pain is?
Pain due to injury/dysfunction in the PNS or CNS
Gate control theory of pain is?
Endogenous modulation e.g. acupuncture e.g. DNIC
Neurones responsible for pain modulation are?
Interneurones in lamina II
DNIC works how? - which fibres and pain pathway are activated?
Diffuse noxious inhibitory control
Activation of a-delta fibres
Stimulation of PAG pathway
PAG pathway involves which two neurotransmitters?
Serotonin
Noradrenaline
PAG pathway involves which other mediator, other than the neurotransmitters?
Opioids
MS lesions are located where?
Periventricular
Three types of pain are?
Nociceptive - acute
Inflammatory - chronic
Neuropathic - chronic maladaptive
What are enkephalines?
Endogenous opioids used in the PAG pathway
Transmitters from NRM to dorsal horn in the PAG pathway are? x2
5-HT
Enkephalines
Transmitter from LC to Dorsal horn in PAG pathway is?
Noradrenaline
Opioids have action at which sites of the PAG pathway? X3
PAG
NRPG
Dorsal horn
Six factors that influence pain perception are?
Cognition Mood Chemicals and structure Context Genetics Injury
Enkepahline are short or long peptides?
Short
Enkephalines are derived from?
Proenkephalin
Opioid receptors are what type of receptor?
G protein coupled receptor