other molecular markers Flashcards
1
Q
what does transcriptomics focus on
A
gene expression
2
Q
what are the steps of transcriptomics
A
- extract mRNA and convert to cDNA
- high throughput sequencing
- align reads to genome
- quantify expression of each gene
3
Q
what is another name for proteomics
A
metabolomics
4
Q
what are the steps of proteomics
A
- extract proteins or metabolites
- digestion and peptide enrichment
- HPLC separation
- mass spectrometry detection