OSPE microscope Flashcards
light microscope
Used to observe living cells, to study their normal behaviors (e.g., migrating or dividing) under the microscope
compound microscope
Used in laboratories, schools, wastewater treatment plants, veterinary offices, and for histology and pathology
Used to view a variety of samples, that includes blood cells, cheek cells, parasites, bacteria, algae, tissue, and thin sections of organs
10x,40x, 100x, 400x
inverted microscope
40x, 100x and sometimes 200x and 400x
analyze and study in-vitro fertilization, live cell imaging, developmental biology, cell biology, neuroscience, and microbiology
fluorescence microscope
Used to image samples that fluoresce (absorb one wavelength of light and emit another)
Light of one wavelength is used to excite the fluorescent molecules, and the light of a different
wavelength that they emit is collected and used to form a picture
must be labeled with a fluorescent dye or tag
To visualize Protozoan parasites, Plasmodium, tubercle bacilli, to detect surface antigens and antibodies
confocal microscope
uses a laser to excite a thin layer of the sample and collects only the emitted light coming from the target layer, producing a sharp image without interference from fluorescent molecules in the surrounding layers
Electron Microscope
Used for detection of viruses, examine not just whole living cells, but also the subcellular structures and compartments within them
Dark field microscope
Used to identify the living, unstained cells and thin bacteria like spirochetes which cannot be visualized by light microscopy
Phase contrast microscope
Used to study living cells and their natural behavior (motility, inner cellular components in detail)