Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

MICROBIO B1 > OSPE culture media > Flashcards

OSPE culture media Flashcards

1
Q

SIMPLE/BASAL MEDIA

A

Contain minimum ingredients that support growth of non-fastidious bacteria
1. Peptone water
2. Nutrient broth
3. Nutrient agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Peptone water

A

cultivation of non- fastidious
bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Nutrient broth

A

Studying bacterial growth curve

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Nutrient agar

A

-Biochemical tests
(oxidase, catalase, slide agglutination test)
* To study colony character

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

ENRICHED MEDIA

A

Addition of nutrients into basal media to Support the growth of fastidious bacteria
1.blood agar
-test haemolytic property

  1. chocolate agar- highly fastidious:H.influenzae,Neisseria meningitidis

3.Loeffler’s serum slope
-corynebacterium diphtheriae isolation

  1. blood culture media- culture blood specimen
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

ENRICHMENT BROTH

A

Liquid media added with some inhibitory agents:

Tetrathionate broth
Gram-negative broth
Selenite F broth
Alkaline peptone water

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Tetrathionate broth

A

Salmonella typhi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Gram-negative broth

A

Shigella, Salmonella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Selenite F broth

A

For the bacteria which cause dysentery ex: Shigella sp.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Alkaline peptone water

A

Vibrio cholerae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

SELECTIVE MEDIA

A

Solid media containing inhibitory substances that inhibit normal flora
1.Loweinstein-Jenson medium
2.Thiosulphate citratae bile salt sucrose agar
3.Deoxycholate agar
4.Xylose lysine deoxycholate agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Loweinstein-Jenson medium

A

Mycobacterium tuberculosis for sputum specimen
jar,green with yellow bumps

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Thiosulfate citrate bile salt sucrose (TCBS) agar

A

Vibrio cholerae from stool specimen
yellow on green agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Deoxycholate citrate (DCA) agar

A

Enteric pathogens: Salmonella-pale medium with black dots,
Shigella from stool specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Xylose lysine deoxycholate (XLD) agar

A

Enteric pathogens: Salmonella-black dots due to H2S , Shigella-pink medium no black from stool specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

SELECTIVE/INDICATOR MEDIA

A

contain an indicator that changes colour when a bacterium grows in them

Wilson and Blair sulphite medium
-green medium S.typhi shows jet black colonies with a metallic sheen are formed due to the production of H2S.

Potassium tellurite agar
Diphtheria bacilli reduce tellurite to metallic tellurium which is incorporated in the colonies, grey or black color

17
Q

DIFFERENTIAL MEDIA

A

Differentiate between two groups of bacteria by using an indicator:

MacConkey agar – isolation of enteric GN bacteria
-Differentiate Lactose fermenter(LF)-(pink-colored colonies) and Non-Lactose Fermenter (NLF)-(colorless colonies)

CLED agar (Cysteine lactose electrolyte –deficient agar)
 For differentiation of LF and NLF of urine specimen
-green

18
Q

TRANSPORT MEDIA

A

Bacteria don’t multiply in the transport media
1. Streptococcus
2. Gonococci
3. Vibrio cholerae
4. For enteric bacilli- Shigella, Salmonella

19
Q

Streptococcus

A

Pike’s medium

20
Q

Gonococci

A

Stuart’s medium

21
Q

Vibrio cholerae

A

VR (Venkatraman-Ramakrishnan) medium, Cary-Blair medium

22
Q

enteric bacilli- Shigella, Salmonella

A

Buffered glycerol saline Cary-Blair medium

23
Q

ANAEROBIC CULTURE MEDIA

A

Contain reducing substances (glutathione) which take up oxygen,
1. Robertson’s cooked meat (RCM) broth

24
Q

Other anaerobic media

A

thioglycolate broth, BHIS agar with supplements (vit K, hemin), Bacteroides bile esculin (BBE) agar, anaerobic blood agar, neomycin blood agar, egg yolk agar, phenylethyl agar

25
Q

other

A
  • GasPack Anaerobic Jar-
    -McIntosh and Flides’ Anaerobic chamber- got P timer
26
Q

STREAK PLATE METHOD

A

Routine method employed for isolation of bacteria

27
Q

LAWN OR CARPET CULTURE

A

Carrying our antimicrobial susceptibility testing by disk diffusion method

28
Q

Stroke culture method-

A

Used are citrate utilization test and urease test

MICROBIO B1 (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions