OSPE culture media Flashcards
SIMPLE/BASAL MEDIA
Contain minimum ingredients that support growth of non-fastidious bacteria
1. Peptone water
2. Nutrient broth
3. Nutrient agar
Peptone water
cultivation of non- fastidious
bacteria
Nutrient broth
Studying bacterial growth curve
Nutrient agar
-Biochemical tests
(oxidase, catalase, slide agglutination test)
* To study colony character
ENRICHED MEDIA
Addition of nutrients into basal media to Support the growth of fastidious bacteria
1.blood agar
-test haemolytic property
- chocolate agar- highly fastidious:H.influenzae,Neisseria meningitidis
3.Loeffler’s serum slope
-corynebacterium diphtheriae isolation
- blood culture media- culture blood specimen
ENRICHMENT BROTH
Liquid media added with some inhibitory agents:
Tetrathionate broth
Gram-negative broth
Selenite F broth
Alkaline peptone water
Tetrathionate broth
Salmonella typhi
Gram-negative broth
Shigella, Salmonella
Selenite F broth
For the bacteria which cause dysentery ex: Shigella sp.
Alkaline peptone water
Vibrio cholerae
SELECTIVE MEDIA
Solid media containing inhibitory substances that inhibit normal flora
1.Loweinstein-Jenson medium
2.Thiosulphate citratae bile salt sucrose agar
3.Deoxycholate agar
4.Xylose lysine deoxycholate agar
Loweinstein-Jenson medium
Mycobacterium tuberculosis for sputum specimen
jar,green with yellow bumps
Thiosulfate citrate bile salt sucrose (TCBS) agar
Vibrio cholerae from stool specimen
yellow on green agar
Deoxycholate citrate (DCA) agar
Enteric pathogens: Salmonella-pale medium with black dots,
Shigella from stool specimen
Xylose lysine deoxycholate (XLD) agar
Enteric pathogens: Salmonella-black dots due to H2S , Shigella-pink medium no black from stool specimen
SELECTIVE/INDICATOR MEDIA
contain an indicator that changes colour when a bacterium grows in them
Wilson and Blair sulphite medium
-green medium S.typhi shows jet black colonies with a metallic sheen are formed due to the production of H2S.
Potassium tellurite agar
Diphtheria bacilli reduce tellurite to metallic tellurium which is incorporated in the colonies, grey or black color
DIFFERENTIAL MEDIA
Differentiate between two groups of bacteria by using an indicator:
MacConkey agar – isolation of enteric GN bacteria
-Differentiate Lactose fermenter(LF)-(pink-colored colonies) and Non-Lactose Fermenter (NLF)-(colorless colonies)
CLED agar (Cysteine lactose electrolyte –deficient agar)
For differentiation of LF and NLF of urine specimen
-green
TRANSPORT MEDIA
Bacteria don’t multiply in the transport media
1. Streptococcus
2. Gonococci
3. Vibrio cholerae
4. For enteric bacilli- Shigella, Salmonella
Streptococcus
Pike’s medium
Gonococci
Stuart’s medium
Vibrio cholerae
VR (Venkatraman-Ramakrishnan) medium, Cary-Blair medium
enteric bacilli- Shigella, Salmonella
Buffered glycerol saline Cary-Blair medium
ANAEROBIC CULTURE MEDIA
Contain reducing substances (glutathione) which take up oxygen,
1. Robertson’s cooked meat (RCM) broth
Other anaerobic media
thioglycolate broth, BHIS agar with supplements (vit K, hemin), Bacteroides bile esculin (BBE) agar, anaerobic blood agar, neomycin blood agar, egg yolk agar, phenylethyl agar
other
- GasPack Anaerobic Jar-
-McIntosh and Flides’ Anaerobic chamber- got P timer
STREAK PLATE METHOD
Routine method employed for isolation of bacteria
LAWN OR CARPET CULTURE
Carrying our antimicrobial susceptibility testing by disk diffusion method
Stroke culture method-
Used are citrate utilization test and urease test
