Organisms: Gram Positive Flashcards
Presumptive ID for:
GPC in clusters
Catalase +
Tube coagulase =
Staphylococcus lugdunesis
Also: PYR +, polymyxin B resistant/rapid ornithine +
May be slide coagulase +/clumpy
Presumptive ID for:
Catalase +
Tube/slide coagulase or latex agglutination +
Staphylococcus aureus
Also: typically B-hemolytic on BAP
Tube coagulase may be required if not hemolytic
Presumptive ID for:
GPC in pairs & chains (no clusters)
Catalase negative
Nonhemolytic colonies >1mm on BAP
Enterococcus spp.
Also: PYR +
If a-hemolytic, also LAP +
Presumptive ID for:
GPC in tetrads & clusters
Catalase =
a-hemolytic
Aerococcus viridans
Also: PYR +, LAP+
Presumptive ID for:
Tiny GPR
Catalase +
Small zone of B-hemolysis on BAP
Usually tumbling motility
Listeria monocytogenes
Also: hippurate +
Should be from blood or CSF
Presumptive ID for:
GPC in pairs & chains
Catalase =
Small B-hemolytic zone on BAP
Streptococcus agalactiae (group B)
Also: hippurate+, rapid CAMP+, Lancefield Group B by latex agglutination
If nonhemolytic, do not use hippurate
Presumptive ID for:
GPC in pairs & chains
Catalase +
<0.5mm Colonies w/ var hemolysis
Streptococcus anginosus | “S. milleri”
Also: odor of butterscotch/vanilla, Lancefield Group F by latex
May also be Group A,C, F, or G by latex
Presumptive ID for:
GP lancet shaped cocci in pairs
Catalase =
a-hemolytic on BAP
Streptococcus pneumoniae
Also: bile solubility +
(Some strains lst solubility to bile)
Presumptive ID for:
GPC in pairs & chains
Catalase =
Sharp B-hemolytic zone on BAP, colony >0.5mm
Streptococcus pyogenes | Group A
Also: PYR +, Lancefield Group A by latex
Look at size & hemolysis to avoid confusion w/ hemolytic enterococci
Presumptive ID for:
GPC in pairs & chains
Catalase =
a-hemolytic or y-hemolytic
Streptococcus, viridans group
Also: PYR =, LAP+, if a-hemolytic bile =
If not in chains: Pediococcus is vanco R, Aerococcus urinae is urine path in tetrads
