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MLS: Microbiology > Organisms: Gram Negative > Flashcards

Organisms: Gram Negative Flashcards

1
Q

Presumptive ID for:
Tiny GN coccobacilli
Oxidase +
Catalase +
No growth on MAC

A

Brucella spp.
Also: urea pos, indolent neg, non-hemolytic

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2
Q

Presumptive ID for:
GNR with gull wings
Oxidase +
Catalase +
Darting motility

A

Campylobacter jejuni/coli
Also: jejuni hippurate +; both indoxyl acetate +
From stool on Campy media at 42C

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3
Q

Presumptive ID from:
Pleomorphic thin GNR
Oxidase +
Catalase =
No growth on MAC

A

Cardidobacterium hominis
Also: indole +, nonhemolytic
Must be from blood culture, confirm nitrate =

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4
Q

Presumptive ID from:
Small GNR
Pitting on BA/CHOC in CO2
Oxidase +
Catalase =
No growth on MAC

A

Eikenella corrodens
Also: indole =, nonhemolytic, bleach odor
Ornithine pos for atypical colonies

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5
Q

Presumptive ID for:
Oxidase =
Indole +
GNR by stain or growing on selective agar

A

Escherichia coli
Also: hemolytic, lactose fermenter, PYR =,
MUG +
Large colonies, not from GI specimens

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6
Q

Presumptive ID for:
Tiny GNR/coccobacilli
Oxidase =
Catalase =/w
Slow growth on Choc, no growth BAP even w/ Staph streak

A

Franscisella tularensis
Also: beta lactamase+
Highly infectious, forward to reference lab

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7
Q

Presumptive ID for:
GN coccobacilli
Gppd growth on choc but not BA/MAC

A

Hemophilius influenzae
Also: ALA=, grows on BAP around Staph (separates from Brucella, Francisella)

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8
Q

Presumptive ID for:
GN coccoid rod
Oxidase +
Catalase =
NG MAC

A

Kingella kingae
Also: hemolytic on BAP, sterile sites

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9
Q

Presumptive ID for:
GN diplococci
Oxidase +
Catalaae +
Entire nonhemolytic colony moves when pushed on BAP

A

Moraxella cararrhalis
Also: butyrate +, indoxyl acetate +

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10
Q

Presumptive ID for:
GN diplococci
Oxidase +
Glistening nonhemolytic growth on BAP

A

Neisseria meningitidis
Also: y-glutamyl-aminopeptidase +
Highly infectious

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11
Q

Presumptive ID for:
GN diplococci
Oxidase +
Grayish nonhemolytic on BAP

A

Neisseria lactamica
Also: B-galactosidase +

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12
Q

Presumptive ID for:
GN diplococci
Oxidase +
Catalase ++ (w/ 30% H2O2)

A

Neisseria gonorrhoeae
Also: y-glutamyl-aminopeptidase =, B-galactosidase =
Must grow on gonococcal agar

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13
Q

Presumptive ID for:
Spreading/swarming colony
Indole =

A

Proteus mirabilis
Also: no other testing if ampicillin susceptible
(If R, is ornithine +/maltose=, P. penneri is reverse)

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14
Q

Presumptive ID for:
Swarming colony
Indole +

A

Proteus vulgaris

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15
Q

Presumptive ID for:
Oxidase +
Indole =

A

Pseudomonas aeruginosa
Also: grape odor
Often strongly B-hemolytic on BAP

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MLS: Microbiology (4 decks)

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