Organisation - enzymes Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

What are enzymes?

A

Large proteins that catalyse reactions.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

How do you work out the rate of reaction?

A

change in mass/ change in time

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the active site?

A

Where the reaction takes place.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is the lock and key theory?

A

The active site is like a lock and the substrate is a key and the active site will only catalyse a specific reaction because the substrate fits into its active site.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What affects the rate of enzyme action?

A

pH and temperature.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How does pH affect the rate of enzyme action?

A

1 If the pH is extreme then the active site changes.
2 Then the substrate can no longer bind to the enzyme.
3 So the enzyme cannot catalyse the reaction - the enzyme is denatured.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

How does temperature affect the rate of enzyme action?

A

1 Increasing temp, increases the reacting activity,
2 Once the enzymes optimum temp is reached the reactivity decreases.
3 The active site changes and the enzyme is denatured and stops working.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are the 3 main digestive enzymes?

A

Amylase, proteases and lipases.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What do digestive enzymes do?

A

Convert food into small, soluble molecules that can be absorbed into the bloodstream.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What do carbohydrases break down carbohydrates into?

A

Simple sugars.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Where is amylase produced?

A

In the salivary glands, pancreas and small intestine.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the catalysed reaction involving amylase?

A

Starch to maltose (simple sugar)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Where are proteases produced?

A

In the stomach, pancreas and small intestine.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the catalysed reaction involving proteases?

A

Proteins into amino acids.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Where is lipase produced?

A

In the pancreas and small intestine.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the catalysed reaction involving lipase?

A

Lipids to fatty acids and glycerol.

17
Q

What is bile?

A

Bile is an alkaline substance that is stored in the gall bladder after having been produced in the liver.

18
Q

What is emulsification?

A

Process of bile breaking up fats into tiny droplets.

19
Q

What does bile also do?

A

Neutralises acid from the stomach to stop enzymes from becoming denatured.

20
Q

How do you test for sugars?

A

1 Add benedict solution to sample and heat for 2 mins.

2 Turns green/yellow/red/brown if sugar is present.

21
Q

How do you test for proteins?

A

1 Add biuret solution.

2 Turns mauve/purple.

22
Q

How do you test for starch?

A

1 Add iodine solution.

2 Solution turns blue/black if starch is present.